Articles
DETERMINATION OF RESISTANCE TO PHYTOPHTHORA CACTORUM CULTURE FILTRATE IN APPLE CLONAL ROOTSTOCKS, CULTIVARS AND LEAF REGENERANTS, USING THE IN VITRO PROLIFERATION AND THE OPTICAL PROBE METHODS.
Article number
336_11
Pages
93 – 100
Language
Abstract
Two methods were tested for early screening of disease resistance in apple rootstocks and cultivars.
The capacity of Phytophthora cactorum culture filtrate (CF) to act as selective agent was tested both on in vitro proliferating shoots and on cell membrane polarization using an optical probe (Merocyanine 540). With both assays, four rootstocks (M.26, MM.106, MM.111, Mark), five cultivars (‘Gala’, ‘Liberty’, ‘McIntosh’, ‘Empire’, ‘Jonathan’) and some M.26 and MM. 106 regenerants were tested.
Both methods were able to characterize different tolerance to CF among the rootstocks, which correlated with their known field resistance.
The second method could be considered more efficient for genotypes discrimination and also for host-pathogen interaction studies.
The capacity of Phytophthora cactorum culture filtrate (CF) to act as selective agent was tested both on in vitro proliferating shoots and on cell membrane polarization using an optical probe (Merocyanine 540). With both assays, four rootstocks (M.26, MM.106, MM.111, Mark), five cultivars (‘Gala’, ‘Liberty’, ‘McIntosh’, ‘Empire’, ‘Jonathan’) and some M.26 and MM. 106 regenerants were tested.
Both methods were able to characterize different tolerance to CF among the rootstocks, which correlated with their known field resistance.
The second method could be considered more efficient for genotypes discrimination and also for host-pathogen interaction studies.
Authors
B. Mezzetti, P. Rosati, R.H. Zimmerman, F.A. Hammerschlag
Keywords
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