Articles
DEVELOPMENT OF A SATURATED LINKAGE MAP OF PRUNUS PERSICA USING MOLECULAR BASED MARKER SYSTEMS
Article number
336_5
Pages
51 – 56
Language
Abstract
Peach [Prunus persica (L.) Batsch.] is considered one of the best genetically characterized fruit tree species.
To develop an understanding of genome organization and to facilitate genetic studies of members of this economically important genus, we chose peach as a model system to generate a high resolution molecular genetic map.
Using low copy genomic clones, 35 restriction fragment length polymorphisms (RFLPs) have been analyzed for segregation in F2 offspring from F1 self-pollinated peach trees originating from a cross between ‘Pillar’ and 77119. Using a statistical program, MAPMAKER, we have developed a genome map consisting of 5 linkage groups with more than one marker, and 10 single unlinked loci.
Additionally, we have used flow cytometry to estimate the genome size of peach to be 6×108 bps, making it one of the smallest plant genomes characterized to date.
To develop an understanding of genome organization and to facilitate genetic studies of members of this economically important genus, we chose peach as a model system to generate a high resolution molecular genetic map.
Using low copy genomic clones, 35 restriction fragment length polymorphisms (RFLPs) have been analyzed for segregation in F2 offspring from F1 self-pollinated peach trees originating from a cross between ‘Pillar’ and 77119. Using a statistical program, MAPMAKER, we have developed a genome map consisting of 5 linkage groups with more than one marker, and 10 single unlinked loci.
Additionally, we have used flow cytometry to estimate the genome size of peach to be 6×108 bps, making it one of the smallest plant genomes characterized to date.
Authors
L.E. Belthoff, R. Ballard, A. Abbott, P. Morgens, A. Callahan, R. Scorza, W.V. Baird, R. Monet
Keywords
Online Articles (52)