Articles
PROPAGATION OF FLOOD TOLERANT JACKFRUIT (ARTOCARPUS HETEROPHYLLUS) BY IN VITRO CULTURE
Article number
336_36
Pages
273 – 278
Language
Abstract
An in vitro culture method for the propagation of flood tolerant jackfruit (Artocarpus heterophyllus) has been achieved.
Jackfruit plants are highly sensitive to water-logging.
Jackfruit is usually propagated by seeds, because budding, grafting and cutting are unsuccessful.
Since the plant is predominantly outbreeding, the characters of the plant differ widely in different individuals.
We have earmarked some flood tolerant individuals and in vitro culture has been employed for their clonal propagation, using shoot buds as explants.
Results showed that addition of 8.88 μM 6-benzyladenine (BA) and 2.68 μM
-naphthaleneacetic acid (NAA) to MS nutrient medium induced maximum number of shoot buds.
These shoots continued to proliferate through 7 or more subcultures with an average of 10 shoots per transfer.
For inducing axial growth in regenerated shoots, the concentrations of BA and NAA were lowered to 4.44 μM and 0.54 μM respectively and coconut milk at 10% (V/V) was added.
For rooting, well-developed shoots were excised and implanted individually on root induction medium.
Within three weeks of transfer, 80% rooting was achieved on a root induction medium consisting of half-strength MS salts supplemented with 5.37 μM NAA and 4.92 μM indolebutyric acid (IBA). Continuous trials using explants from the elite trees throughout the year showed that the period between June-August was the best season for taking explants to insure rapid and increased multiplication of axillary buds.
Young rooted plantlets were transplanted directly from the culture tube to earthen pots containing sterile sand, soil and humus (1:2:1) and covered by transparent plastic bags.
After acclimatization the potted plants were flooded with water for 30 days; 75% of these plants survived.
Jackfruit plants are highly sensitive to water-logging.
Jackfruit is usually propagated by seeds, because budding, grafting and cutting are unsuccessful.
Since the plant is predominantly outbreeding, the characters of the plant differ widely in different individuals.
We have earmarked some flood tolerant individuals and in vitro culture has been employed for their clonal propagation, using shoot buds as explants.
Results showed that addition of 8.88 μM 6-benzyladenine (BA) and 2.68 μM
-naphthaleneacetic acid (NAA) to MS nutrient medium induced maximum number of shoot buds.These shoots continued to proliferate through 7 or more subcultures with an average of 10 shoots per transfer.
For inducing axial growth in regenerated shoots, the concentrations of BA and NAA were lowered to 4.44 μM and 0.54 μM respectively and coconut milk at 10% (V/V) was added.
For rooting, well-developed shoots were excised and implanted individually on root induction medium.
Within three weeks of transfer, 80% rooting was achieved on a root induction medium consisting of half-strength MS salts supplemented with 5.37 μM NAA and 4.92 μM indolebutyric acid (IBA). Continuous trials using explants from the elite trees throughout the year showed that the period between June-August was the best season for taking explants to insure rapid and increased multiplication of axillary buds.
Young rooted plantlets were transplanted directly from the culture tube to earthen pots containing sterile sand, soil and humus (1:2:1) and covered by transparent plastic bags.
After acclimatization the potted plants were flooded with water for 30 days; 75% of these plants survived.
Authors
S.K. Roy, M.S. Islam, J. Sen, A.B.M.E. Hossain, S. Hadiuzzaman
Keywords
Shoot culture, fruit plant, propagation, auxins, cytokinin
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