Articles
TOWARDS THE SOMATIC HYBRIDIZATION OF SHRUBBY AND CLIMBING HONEYSUCKLES
Article number
336_43
Pages
327 – 332
Language
Abstract
The genus Lonicera includes several shrubby (with small, unperfumed flowers) and climbing (with big, scented flowers) species that are among the most widely cultivated ornamentals in Europe.
It would be desirable to create shrubby hybrids with big perfumed flowers.
This goal, though, has proved elusive to date on account of incompatibility problems that preclude natural natural gene flow betwen species.
Protoplast fusion, with the further recovery of novel somatic hybrids, appeared as a feasible alternative to overcome the existing barriers to sexual crossing.
However, the development of protoplast-to-plant systems for the prospective fusion partners is a prerequisite for such goal.
Against this background, large numbers (> 106/g f.w.) of highly viable (> 80%) protoplasts were isolated from in vitro leaves, callus tissues and cell suspensions of the shrubby species L. fragrantissima and the climbing genotypes L. periclymenum ‘Serotina’, and L. x brownii ‘Dropmore Scarlet’. The requirements for protoplast isolation for each genotype and source tissue were established.
The cultured protoplasts divided to produce small cell colonies, and then microcalluses.
The percentage plating efficiency, at various developmental stages, was assessed for protoplasts of all genotypes and sources.
Experiments aimed at the further growth and subsequent organogenesis from such protoplast-derivd honeysuckle tissues are now underway.
It would be desirable to create shrubby hybrids with big perfumed flowers.
This goal, though, has proved elusive to date on account of incompatibility problems that preclude natural natural gene flow betwen species.
Protoplast fusion, with the further recovery of novel somatic hybrids, appeared as a feasible alternative to overcome the existing barriers to sexual crossing.
However, the development of protoplast-to-plant systems for the prospective fusion partners is a prerequisite for such goal.
Against this background, large numbers (> 106/g f.w.) of highly viable (> 80%) protoplasts were isolated from in vitro leaves, callus tissues and cell suspensions of the shrubby species L. fragrantissima and the climbing genotypes L. periclymenum ‘Serotina’, and L. x brownii ‘Dropmore Scarlet’. The requirements for protoplast isolation for each genotype and source tissue were established.
The cultured protoplasts divided to produce small cell colonies, and then microcalluses.
The percentage plating efficiency, at various developmental stages, was assessed for protoplasts of all genotypes and sources.
Experiments aimed at the further growth and subsequent organogenesis from such protoplast-derivd honeysuckle tissues are now underway.
Authors
D. Georges, L. Decourtye, S.J. Ochatt
Keywords
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