Articles
CELL SUSPENSION CULTURES IN STRAWBERY: GROWTH CHARACTERIZATION AND VARIABILITY
Article number
336_33
Pages
257 – 262
Language
Abstract
An efficient and suitable protocol for cell suspension culture establishment has been defined in the strawberry cultivar Pajaro.
Growth and viability characteristics of cells in culture have been analyzed; the sigmoid curve is defined in 20 days of culture, with an initial optimal concentration of 5ml/20 ml (cells/medium). The best medium was composed of B5 Gamborg salts supplemented with 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Preliminary studies on isoperoxidase activity are also presented; cathodal isoperoxidases did not show any polymorphism related to the growth of cells.
Growth and viability characteristics of cells in culture have been analyzed; the sigmoid curve is defined in 20 days of culture, with an initial optimal concentration of 5ml/20 ml (cells/medium). The best medium was composed of B5 Gamborg salts supplemented with 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Preliminary studies on isoperoxidase activity are also presented; cathodal isoperoxidases did not show any polymorphism related to the growth of cells.
Authors
F. Blando, A. Niglio, A. Frattarelli, S. Speranza, C. Damiano
Keywords
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