EFFECTS OF NACL AND CACL₂ IN PRUNUS CERASIFERA TISSUE CULTURE

The response of in vitro-grown Prunus cerasifera plantlets was assayed when increasing NaCl doses (35, 100, 150, 250 mM) in combination with two CaCl₂ concentrations (3, 10 mM) were added to the medium (LS). Negative effects were observed at 150 and 250 mM salt doses in combination with 3 mM CaCl₂. Marked improvement was obtained when 10 mM CaCl₂ was added to the medium, confirming that Ca⁺⁺ acts as a cell membrane protector against the adverse effects of Na⁺. Results also indicated that plantlets were not affected by salinity at 35 mM salt concentration in combination with 3 mM CaCl₂. Predominant effects at this dose were probably osmotic, due to greater Na⁺ availability.

II International Symposium on In Vitro Culture and Horticultural Breeding

M. Lucchesini, C. Vitagliano

https://doi.org/10.17660/ActaHortic.1993.336.13