Articles
WOODY PLANT PROTOPLAST TECHNOLOGY REVISITED
Article number
336_38
Pages
285 – 296
Language
Abstract
After nearly three decades of basic protoplast research, it is now clear that the potential for economic gain through protoplast manipulation will finally be fulffilled and the technology progressively accepted by plant breeders and seed companies.
Progress in the application of protoplast technology to the improvement of cultivated crops is still somewhat restricted by a lack of plant regeneration in some species despite notable advances, in recent times, with respect to the cereal and woody species.
Interestingly, though, the small fruit crops (notably the grapevine), many vegetable crops and species of floral value, the monocotyledonous species and most woody plant genotypes are in general difficult to deal with, and the establishment of reproducible protoplast-to-plant systems is still largely achieved by empirical means.
There is a compelling case to increase our knowledge as to the role that complex media play in triggering sustained division in protoplast-derived cells, and also of the relationships between factors and the genetic basis to plant regeneration.
This paper will summarize and critically examine recent advances in protoplast technology within the context of breeding holticultural crops and, mainly, as applied to deciduous temperature fruit tree species.
Progress in the application of protoplast technology to the improvement of cultivated crops is still somewhat restricted by a lack of plant regeneration in some species despite notable advances, in recent times, with respect to the cereal and woody species.
Interestingly, though, the small fruit crops (notably the grapevine), many vegetable crops and species of floral value, the monocotyledonous species and most woody plant genotypes are in general difficult to deal with, and the establishment of reproducible protoplast-to-plant systems is still largely achieved by empirical means.
There is a compelling case to increase our knowledge as to the role that complex media play in triggering sustained division in protoplast-derived cells, and also of the relationships between factors and the genetic basis to plant regeneration.
This paper will summarize and critically examine recent advances in protoplast technology within the context of breeding holticultural crops and, mainly, as applied to deciduous temperature fruit tree species.
Authors
S.J. Ochatt
Keywords
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