Articles
SOMATIC HYBRIDIZATION BETWEEN SENECIO FUSCHII GMEL. AND SENECIO JACOBAEA L.
Article number
336_41
Pages
315 – 320
Language
Abstract
Somatic hybridization between Senecio fuchsii Gmel. and two albino mutants (one plastid and one nuclear mutant) of S. jacobaea L. was investigated.
Fusion was obtained via the agarose sandwich lenses technique with high Ca++ and high pH. A series of regenerant lines were selected by pigmentation and morphological charateristics.
The constitutions of five hybrid and two cybrid lines of ten lines examined were characterized as to chromosome numbers, isozyme patterns (peroxidase, estrase, malate dehydrogenase, glutamic-oxalacetic transaminase), and RFLPs.
Restriction fragment patterns of total DNA were explored by specific probes of nuclear DNA and chloroplast DNA. Plastids has segregated in the shoots investigated.
The two cybrids each possessed 2n=2x=40 chromosomes.
One contained the S. jacobaea nuclear genome and S. fuchsii plastome, the other contained S. fuchsii nuclear genome and S. jacobaea plastome.
Analyses of RFLPs with mtDNA probes are progress.
The development of plants in the greenhouse is being followed.
Fusion was obtained via the agarose sandwich lenses technique with high Ca++ and high pH. A series of regenerant lines were selected by pigmentation and morphological charateristics.
The constitutions of five hybrid and two cybrid lines of ten lines examined were characterized as to chromosome numbers, isozyme patterns (peroxidase, estrase, malate dehydrogenase, glutamic-oxalacetic transaminase), and RFLPs.
Restriction fragment patterns of total DNA were explored by specific probes of nuclear DNA and chloroplast DNA. Plastids has segregated in the shoots investigated.
The two cybrids each possessed 2n=2x=40 chromosomes.
One contained the S. jacobaea nuclear genome and S. fuchsii plastome, the other contained S. fuchsii nuclear genome and S. jacobaea plastome.
Analyses of RFLPs with mtDNA probes are progress.
The development of plants in the greenhouse is being followed.
Authors
G.R. Wang, H. Binding
Keywords
Somatic hybridization, Senecio fuchsii Gmel., Senecio jacobaea L, RFLPs, hybrids, cybrids
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