Articles
INCORPORATION OF THE GUS GENE INTO ORCHIDS THROUGH EMBRYO ELECTROPHORESIS
Article number
336_20
Pages
165 – 170
Language
Abstract
Pollen and the apical meristems of Calanthe orchid embryos were exposed to DNA under various treatments and conditions.
The only treatment that produced transgenic plants was embryo electrophoresis.
A pipette containing DNA suspended in agarose dissolved in acetate buffer was placed on the apical meristem of the embryo, while the opposite end of the embryo was in contact with a pipette containing only buffer.
The optimal conditions for gene transfer were at 50 volts and 0.5 milliampere for 10 min.
Under these conditions, 55% of the embryos survived the treatment and 57% of those that survived were transformed.
The only treatment that produced transgenic plants was embryo electrophoresis.
A pipette containing DNA suspended in agarose dissolved in acetate buffer was placed on the apical meristem of the embryo, while the opposite end of the embryo was in contact with a pipette containing only buffer.
The optimal conditions for gene transfer were at 50 volts and 0.5 milliampere for 10 min.
Under these conditions, 55% of the embryos survived the treatment and 57% of those that survived were transformed.
Authors
R.J. Griesbach, J. Hammond
Keywords
Calanthe, transformation, transgenic plant
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