Articles
PRODUCTION OF GENETICALLY ENGINEERED COLOR-MODIFIED CHRYSANTHEMUM PLANTS CARRYING A HOMOLOGOUS CHALCONE SYNTHASE GENE AND THEIR FIELD PERFORMANCE
Article number
336_6
Pages
57 – 62
Language
Abstract
We introduced a chimeric chalcone synthase (CHS) gene isolated from chrysanthemum (Dendranthema grandiflora) into cv.
Moneymaker (a pink decorative anemone-centered type), via Agrobacterium-mediated leaf disk transformation, to produce white-flowered plants.
The CHS coding sequence was in either antisense or sense orientation relative to the CAMV 35S promoter.
Some 3.6% (3/83) of the antisense-transformed plants and 1.5% (2/133) of the sense-transformed plants produced completely white flowers.
Pigment analysis revealed that this is due to a block at CHS. To study stability of color change and horticultural characteristics of the white ‘Moneymaker’ plants, field trials were carried out.
Four lines were compared: ‘Moneymaker’, a ‘Moneymaker’ regenerant, an antisense white (2706), and a sense white (31435). There was no difference between ‘Moneymaker’ and the regenerant.
Both 2706 and 31435 were vegetatively propagated with good stability; all plants produced white or very pale pink flowers.
The proportion of plants producing pale pink flowers varied among field trial locations.
Line 2706 flowered about 7 days later than the control plants and line 31435 about 10-12 days later than controls.
Flower number was similar for all four lines tested.
Moneymaker (a pink decorative anemone-centered type), via Agrobacterium-mediated leaf disk transformation, to produce white-flowered plants.
The CHS coding sequence was in either antisense or sense orientation relative to the CAMV 35S promoter.
Some 3.6% (3/83) of the antisense-transformed plants and 1.5% (2/133) of the sense-transformed plants produced completely white flowers.
Pigment analysis revealed that this is due to a block at CHS. To study stability of color change and horticultural characteristics of the white ‘Moneymaker’ plants, field trials were carried out.
Four lines were compared: ‘Moneymaker’, a ‘Moneymaker’ regenerant, an antisense white (2706), and a sense white (31435). There was no difference between ‘Moneymaker’ and the regenerant.
Both 2706 and 31435 were vegetatively propagated with good stability; all plants produced white or very pale pink flowers.
The proportion of plants producing pale pink flowers varied among field trial locations.
Line 2706 flowered about 7 days later than the control plants and line 31435 about 10-12 days later than controls.
Flower number was similar for all four lines tested.
Authors
N. Courtney-Gutterson, E. Firoozabady, C. Lemieux, J. Nicholas, A. Morgan, K. Robinson, A. Otten, M. Akerboom
Keywords
Dendranthema grandiflora, Agrobacterium tumefaciens, cosuppression, antisense, transformation
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