Articles
IN VITRO MICROPROPAGATION OF INDICATOR PLANTS FOR INDEXING PRUNUS NECROTIC RING SPOT VIRUS
Article number
336_15
Pages
121 – 126
Language
Abstract
The objective of the present study was to develop an in vitro system for propagation of plant indicators to Prunus necrotic ring spot virus (PNRSV) as a model for shortening the time of indexing.
Two plant indicators were micropropagated: Prunus tomentosa and P. serrulata ‘Shirofugen’. The media for shoot culture establishment were Almehdi and Parfitt (AP) containing 0.89 μM benzyladenine (BA) and 0.05 μM indolebutyric acid (IBA), for P. tomentosa, and Boxus medium containing 4.4 μM BA, 0.029 μM gibberellic acid (GA3) and 0.09 μM 2,4-dichlorophenoxyacetic acid (2,4-D) for ‘Shirofugen’. The media for shoot proliferation were AP + 0.05 μM IBA, 2.2 μM BA and 0.58 μM GA3 for ‘Shirofugen’ and AP + 0.05 μM IBA and 0.89 μM BA for P. tomentosa. The rooting medium was half-strength Murashige and Skoog medium containing 2.7 μM naphthaleneacetic acid (NAA) for P. tomentosa and 4.9 μM IBA for ‘Shirofugen’. PNRSV-infected peach cultures were reciprocally grafted in sterile conditions on indicators.
Virus transmission to the indicators has been detected by the ELISA method.
Two plant indicators were micropropagated: Prunus tomentosa and P. serrulata ‘Shirofugen’. The media for shoot culture establishment were Almehdi and Parfitt (AP) containing 0.89 μM benzyladenine (BA) and 0.05 μM indolebutyric acid (IBA), for P. tomentosa, and Boxus medium containing 4.4 μM BA, 0.029 μM gibberellic acid (GA3) and 0.09 μM 2,4-dichlorophenoxyacetic acid (2,4-D) for ‘Shirofugen’. The media for shoot proliferation were AP + 0.05 μM IBA, 2.2 μM BA and 0.58 μM GA3 for ‘Shirofugen’ and AP + 0.05 μM IBA and 0.89 μM BA for P. tomentosa. The rooting medium was half-strength Murashige and Skoog medium containing 2.7 μM naphthaleneacetic acid (NAA) for P. tomentosa and 4.9 μM IBA for ‘Shirofugen’. PNRSV-infected peach cultures were reciprocally grafted in sterile conditions on indicators.
Virus transmission to the indicators has been detected by the ELISA method.
Authors
S. Zilkah, E. Faingersh, A. Rotbaum, A. Stein
Keywords
‘Shirofugen’ (Prunus serrulata), Prunus tomentosa, tissue culture, quarantine
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