CLASSIFICATION OF CULTIVARS OF PHASEOLUS VULGARIS ACCORDING TO THEIR SDS-PAGE PROTEIN PATTERNS

Forty cultivars of Phaseolus vulgaris at collection of IWS "Dobroudja" are subjected to investigation with the purpose for increasing the effectiveness of the hybridization.
In the study a sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS – PAGE) of seed storage protein is used.
Individual electrophoregrams of the cultivars can be used as pass-port data for their genetic identity.
Using cluster – analysis, all cultivars are arranged in ten different groups.
The results show, that the level of index similarity between cultivars is from 59 to 98. The cultivars Cher Starozagorski and WA 4497-3 are suitable gene sources for high protein content.
Genetically they are sufficiently different Cher Starozagorski is in the fourth cluster, WA 4497-3 is in the seventh cluster.
Cultivars classification based on protein polymorphism is not very similar with the differences found in the biomorpho-logical clustering.
This classification can be used for correct choice of genotypes for combining selection.

I Balkan Symposium On Vegetables and Potatoes

Milka V. Stoyanova

bean cultivars, protein, electrophoresis, dendrogram

https://doi.org/10.17660/ActaHortic.1997.462.137