Articles
IN VITRO SELECTION FOR RESISTANCE TO FUSARIUM OXYSPORUM F. SP. DIANTHI IN CARNATION
Article number
482_45
Pages
309 – 314
Language
Abstract
Carnation leaf explants from micropropagated plantlets cv.
Nora Barlo were used to obtain adventitious regeneration and to select plantlets resistant to Fusarium oxysporum f. sp. dianthi. Leaf pieces from the basal third part of the leaves were cultivated on Murashige & Skoog (MS) medium supplemented with 1 mg/L of 6-benzylaminopurine (BAP) and 1 mg/L naphthalene acetic acid NAA). An isolate of F. oxysporum f. sp. dianthi race 2 was grown in liquid hydrolized casein medium for seven days at 22°C. The culture was then autoclaved and homogenized in a blender.
This homogenate was added to the medium for adventitious regeneration in concentrations from 0% to 40% (v/v). At the highest concentrations, there were not calluses induction and plantlets regeneration.
There was callus induction on medium up to 10% of fungus homogenate and there was plantlets adventitious regeneration on medium up to 5%. These plantlets showed good morphological and physiological characteristics such as vegetative vigor and fast regeneration.
Nora Barlo were used to obtain adventitious regeneration and to select plantlets resistant to Fusarium oxysporum f. sp. dianthi. Leaf pieces from the basal third part of the leaves were cultivated on Murashige & Skoog (MS) medium supplemented with 1 mg/L of 6-benzylaminopurine (BAP) and 1 mg/L naphthalene acetic acid NAA). An isolate of F. oxysporum f. sp. dianthi race 2 was grown in liquid hydrolized casein medium for seven days at 22°C. The culture was then autoclaved and homogenized in a blender.
This homogenate was added to the medium for adventitious regeneration in concentrations from 0% to 40% (v/v). At the highest concentrations, there were not calluses induction and plantlets regeneration.
There was callus induction on medium up to 10% of fungus homogenate and there was plantlets adventitious regeneration on medium up to 5%. These plantlets showed good morphological and physiological characteristics such as vegetative vigor and fast regeneration.
Authors
T. Mosquera, L.E. Rodríguez, C. Bernal, F. López
Keywords
Online Articles (61)