Articles
A LOW COST STRATEGY FOR MICROPROPAGATION OF STRAWBERRY (FRAGARIA × ANANASSA DUCH.) CV. CHANDLER
Article number
696_22
Pages
129 – 133
Language
English
Abstract
A low cost medium in vitro propagation of strawberry (Fragaria × ananassa Duch) cv.
Chandler was developed.
Vegetative buds were cultured on Murashige and Skoog (MS) medium supplemented with low cost medium components.
The major medium manipulations were made by replacing agar-agar, sucrose and distilled water with tapioca granules, table sugar and tap water, respectively.
Maximum in vitro multiplication of shoots was obtained on MS medium supplemented with kn 0.5 mg/l, BAP 1.0 mg/l and GA3 2.0 mg/l and table sugar in place of sucrose and maximum in vitro rooting was induced on one-fourth MS medium supplemented with IBA 1.0 mg/l, charcoal 200 mg/l and table sugar 20.0 g/l in place of sucrose.
Hundred per cent survival of micropropagated plants was recorded in the field.
Plants grown through cost effective micropropagated protocol showed good field adaptability.
Chandler was developed.
Vegetative buds were cultured on Murashige and Skoog (MS) medium supplemented with low cost medium components.
The major medium manipulations were made by replacing agar-agar, sucrose and distilled water with tapioca granules, table sugar and tap water, respectively.
Maximum in vitro multiplication of shoots was obtained on MS medium supplemented with kn 0.5 mg/l, BAP 1.0 mg/l and GA3 2.0 mg/l and table sugar in place of sucrose and maximum in vitro rooting was induced on one-fourth MS medium supplemented with IBA 1.0 mg/l, charcoal 200 mg/l and table sugar 20.0 g/l in place of sucrose.
Hundred per cent survival of micropropagated plants was recorded in the field.
Plants grown through cost effective micropropagated protocol showed good field adaptability.
Publication
Authors
R. Kaur, H. Gautam, D.R. Sharma
Keywords
strawberry, cost effective, micropropagation
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