Articles

MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY IN FRAGARIA GENOTYPES AS REVEALED BY RANDOMLY AMPLIFIED DNA POLYMORPHISMS (RAPDS)

Article number
696_23
Pages
135 – 142
Language
English
Abstract
The efficiency of RAPD markers for Fragaria genotype DNA fingerprinting and the estimation of genetic relatedness was evaluated.
Thirteen cultivars of Fragaria × ananassa Duch. and two wild species, Fragaria indica Andr. and Fragaria vesca Linn. were analyzed with twenty 10-bp primers.
Only thirteen yielded scorable polymorphic amplification patterns based on discernible bands, however, no single primer out of these thirteen could produce a unique fingerprint for all the fifteen genotypes.
Cluster analysis based on UPGMA separated the fifteen Fragaria genotypes into two major groups, with the thirteen cultivars and Fragaria indica in a group separate from Fragaria vesca. The mean genetic distance among the genotypes was 36 % implying that large part of the genome is not similar among the genotypes.
Genetic diversity studies could not be related to pedigrees due to a lack of information about the same.

Publication
Authors
S. Kashyap, R. Kaur, D.R. Sharma, K. Kumar, S.K. Sharma
Keywords
strawberry, DNA fingerprinting, genetic relatedness, cluster analysis
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