Articles
ISSR MARKERS LINKED TO THE TWO GENES DETERMINING INDETERMINATE GROWTH HABIT (FIN) AND SEEDS’ COLOR (P) IN COMMON BEAN
Article number
830_13
Pages
107 – 114
Language
English
Abstract
Two bulked DNA samples of segregating population obtained by single cross (A 195 x Kristal 137) were prepared for bulked segregant analysis (BSA). Each pool, or bulk, contained identical individuals of particular trait or genomic region dispersed arbitrary at all unlinked regions.
BSA was used to identify inter simple sequence repeats (ISSR) markers linked to the genes determining indeterminate growth habit (Fin) and seeds color (P) in F2 progeny.
Cultivar A 195 has determinate growth habit and brown seeds, while Kristal 137 has indeterminate growth habit and white seeds.
Two different bulked DNAs were prepared from equal volumes of standardized DNA of those plants.
Among the 72 ISSR primers used to discriminate the two parental genotypes and between the two DNA bulks (determinate versus indeterminate), only 32 (44.44%) amplified polymorphic markers.
Previously confirmed 10 primers amplified clear polymorphic bands.
They were further tested among 100 F2 plants.
The analysis of the genetic linkage between analyzed molecular markers and the loci for indeterminate growth habit and seeds color was performed using the program Join Map 3.0 (Van Ooijen and Voorrips, 2001). The logarithm of odds (LOD) of 3.0 was used as a linkage threshold for linkage grouping.
Map distances (cM) between loci were calculated based on recombination coefficient values using the Kosambis mapping function (Kosambi, 1944). ISSR marker L11, at distance 40.9 cM from the P gene (LODlinkage 3.21) and 41.5 cM from the gene Fin (LODlinkage 3.48), was mapped.
ISSR marker L8 was also mapped to the Fin gene on distance 44.1 cM (LODlinkage 3.10). The coupling-phase ISSR markers detected in that study could be used to select for some levels of quantitative traits associated with Fin and P genes in common bean.
Investigations have to continue for discovering more closely related ISSR markers with Fin and P genes.
BSA was used to identify inter simple sequence repeats (ISSR) markers linked to the genes determining indeterminate growth habit (Fin) and seeds color (P) in F2 progeny.
Cultivar A 195 has determinate growth habit and brown seeds, while Kristal 137 has indeterminate growth habit and white seeds.
Two different bulked DNAs were prepared from equal volumes of standardized DNA of those plants.
Among the 72 ISSR primers used to discriminate the two parental genotypes and between the two DNA bulks (determinate versus indeterminate), only 32 (44.44%) amplified polymorphic markers.
Previously confirmed 10 primers amplified clear polymorphic bands.
They were further tested among 100 F2 plants.
The analysis of the genetic linkage between analyzed molecular markers and the loci for indeterminate growth habit and seeds color was performed using the program Join Map 3.0 (Van Ooijen and Voorrips, 2001). The logarithm of odds (LOD) of 3.0 was used as a linkage threshold for linkage grouping.
Map distances (cM) between loci were calculated based on recombination coefficient values using the Kosambis mapping function (Kosambi, 1944). ISSR marker L11, at distance 40.9 cM from the P gene (LODlinkage 3.21) and 41.5 cM from the gene Fin (LODlinkage 3.48), was mapped.
ISSR marker L8 was also mapped to the Fin gene on distance 44.1 cM (LODlinkage 3.10). The coupling-phase ISSR markers detected in that study could be used to select for some levels of quantitative traits associated with Fin and P genes in common bean.
Investigations have to continue for discovering more closely related ISSR markers with Fin and P genes.
Publication
Authors
E. Apostolova, D. Svetleva
Keywords
Phaseolus vulgaris L., molecular markers, bulcked segregant analysis, Inter Simple Sequence Repeats, map distance
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