MAINTENANCE AND IN VITRO CONSERVATION OF POTATOES

In vitro conservation of asexually reproduced plant species gives an alternative way to maintain collections of plant genetic material.
Different factors affecting the growth rate of tissue cultures in vitro conditions and conservation period have been investigated.
Explants from different cultivars were cultivated on media supplemented with growth inhibitors as Alar and ABA, poor nutrient media and temperature regimes.
The conducted experiment with addition of plant inhibitors in nutrient media established Alar – 50 g/L and ABA – 5 mg/L as more appropriated for long term conservation of the investigated cultivars.
It has been found that the most appropriate preserving temperatures are 8 and 10°C. Different cultivars show different abilities for tuber formation in vitro.
Cultivars Aquila and Gamma are characterized by high level of viability after one year conservation.
They recover normal growth when cultivated on optimal conditions in fresh nutrient medium.

IV Balkan Symposium on Vegetables and Potatoes

D. Dimitrova, M.P. Marcheva

tissue culture, growth regulator, temperature, inhibitor, genetic resources

https://doi.org/10.17660/ActaHortic.2009.830.7