Articles
MORPHOLOGICAL AND GENETIC VARIABILITY IN SOME ARTEMISIA SPECIES
Article number
830_99
Pages
687 – 694
Language
English
Abstract
The aim of this study was to determine the morphological and genetic variability of five species of Artemisia: southernwood (A. abrotanum L.), tarragon (A. dracunculus L.), mugwort (A. absinthium L.), roman wormwood (A. pontica L.), and common wormwood (A. vulgaris L.) from the Collection of Medical Plants of the Vegetable Department of the University of Agricultural in Szczecin.
Morphological variation among the Artemisia plants was determined using biometric measurements, which included the growth dynamics, the height of the plants, the width of a leaf pair, the length and width of a leaf blade.
ISSR amplification was used to analyze polymorphisms of microsatellite sequences in the Artemisia genome and to evaluate genetic diversity among them.
Forty microsatellite primers were used in ISSR reactions.
Clear products were generated in reactions with fifteen of them.
In general, 120 loci were amplified (eight of the average); five of them 71 turned out to be polymorphic, and 49 specific for the examined species.
The longest ISSR products (~3000 bp) was amplified with the 854 primer and the shortest ((~220 bp) with the 810 primer.
Analysis of the phylogenetic similarity dendrogram has shown wide range of diversity between studied species, which was from 3,6% to 56,7%. The biggest genetic similarity (56,7%) characterized A. absinthium L. and A. dracunculus L. species.
Phylogenetic similarity A. pontica L. and A. abrotanum L. come to 15%, whereas A. vulgaris L. and A. dracunculus L. come to 49,6%.
Morphological variation among the Artemisia plants was determined using biometric measurements, which included the growth dynamics, the height of the plants, the width of a leaf pair, the length and width of a leaf blade.
ISSR amplification was used to analyze polymorphisms of microsatellite sequences in the Artemisia genome and to evaluate genetic diversity among them.
Forty microsatellite primers were used in ISSR reactions.
Clear products were generated in reactions with fifteen of them.
In general, 120 loci were amplified (eight of the average); five of them 71 turned out to be polymorphic, and 49 specific for the examined species.
The longest ISSR products (~3000 bp) was amplified with the 854 primer and the shortest ((~220 bp) with the 810 primer.
Analysis of the phylogenetic similarity dendrogram has shown wide range of diversity between studied species, which was from 3,6% to 56,7%. The biggest genetic similarity (56,7%) characterized A. absinthium L. and A. dracunculus L. species.
Phylogenetic similarity A. pontica L. and A. abrotanum L. come to 15%, whereas A. vulgaris L. and A. dracunculus L. come to 49,6%.
Publication
Authors
D. Rzepka-Plevne, M. Smolik, K. Urbanek, D. Jadczak
Keywords
Artemisia species, variability, morphology, genetic, molecular markers
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