Articles
ANTIOXIDANT ACTIVITIES AND HPLC/UV/MS CHARACTERIZATION OF FLAVONOID EXTRACTS FROM OPUNTIA FICUS INDICA SEEDS AND PEELS
Article number
853_22
Pages
189 – 198
Language
English
Abstract
Methanol extracts of Opuntia ficus indica seeds and peels were fractionated and their subfractions were studied by high performance liquid chromatography (HPLC)–ultraviolet detectionatmospheric pressure chemical ionisation (APCI) mass spectrometry (MS) for the first time.
Eleven flavonoids are identified in the methanol peels extract.
Flavonic aglycones belong to flavonols, dihydroflavonols and flavanones.
The major glycosylated flavonoids are isorhamnetin mono-, di- and tri-glucosides.
Four flavonoids were identified in the seeds extract as prenyl flavanones and methoylated flavones.
Aglycones and monoglucosides were the major compounds of these extracts.
Sugars were identified after extracts acid hydrolysis by comparison with standards.
The antioxidant activities of both methanolic extracts were determined with DPPH assay, ABTS test and reducing power.
All methods showed the seeds extract is a powerful scavenger.
Eleven flavonoids are identified in the methanol peels extract.
Flavonic aglycones belong to flavonols, dihydroflavonols and flavanones.
The major glycosylated flavonoids are isorhamnetin mono-, di- and tri-glucosides.
Four flavonoids were identified in the seeds extract as prenyl flavanones and methoylated flavones.
Aglycones and monoglucosides were the major compounds of these extracts.
Sugars were identified after extracts acid hydrolysis by comparison with standards.
The antioxidant activities of both methanolic extracts were determined with DPPH assay, ABTS test and reducing power.
All methods showed the seeds extract is a powerful scavenger.
Authors
I. El Mannoubi, S. Barrek, T. Skanji, H. Zarrouk
Keywords
antioxidants, by-products, flavonoids, high performance liquid chromatography, mass spectrometry, prickly pear cactus
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