Articles
APPLICATION OF ALTERNATIVE LOADING SOLUTIONS TO GARLIC AND CHRYSANTHEMUM IN DROPLET-VITRIFICATION PROCEDURES
Article number
908_19
Pages
173 – 180
Language
English
Abstract
In plant vitrification protocols, the loading treatment, i.e. exposure of explants to a moderately concentrated cryoprotectant solution, precedes the main dehydration step with highly concentrated vitrification solutions in order to reduce their toxicity.
This study aimed at developing alternative loading solutions composed of glycerol and sucrose mixed at various concentrations.
The solutions were applied to two model species, garlic and chrysanthemum, cryopreserved via a droplet-vitrification procedure.
Differential scanning calorimetry measurements of loading solutions and of loaded and dehydrated explants were performed to assay thermal events occurring during cooling and warming.
The response to the loading solutions varied between the two species.
In garlic apices, all the loading solutions produced a similar effect, whereas recovery of chrysanthemum shoot tips was significantly influenced by the solution composition.
A loading solution comprising of 1.9 M glycerol and 0.5 M sucrose was the most efficient.
The loading treatment may thus act as an osmotic stress neutralizer and/or contribute to the physiological adaptation of tissues to both dehydration and freezing.
This study aimed at developing alternative loading solutions composed of glycerol and sucrose mixed at various concentrations.
The solutions were applied to two model species, garlic and chrysanthemum, cryopreserved via a droplet-vitrification procedure.
Differential scanning calorimetry measurements of loading solutions and of loaded and dehydrated explants were performed to assay thermal events occurring during cooling and warming.
The response to the loading solutions varied between the two species.
In garlic apices, all the loading solutions produced a similar effect, whereas recovery of chrysanthemum shoot tips was significantly influenced by the solution composition.
A loading solution comprising of 1.9 M glycerol and 0.5 M sucrose was the most efficient.
The loading treatment may thus act as an osmotic stress neutralizer and/or contribute to the physiological adaptation of tissues to both dehydration and freezing.
Authors
H.-H. Kim, E. Popova, N.-Y. No, H.-J. Baek, C.-K. Kim, E.-G. Cho, F. Engelmann
Keywords
chrysanthemum, differential scanning calorimetry, droplet-vitrification, garlic, loading solution, shoot tips
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