CONSERVATION STRATEGY OF GENETIC RESOURCES FOR STRAWBERRY IN GERMANY

The National German Strawberry Genebank includes 369 cultivars and the active field collection in Dresden-Pillnitz also contains 317 Fragaria wild species accessions.
The conservation strategy requires the application of several methods for safety duplication.
First, in vitro cold storage was developed and adapted.
The average storage duration at 4°C for a range of strawberry cultivars was 22 months using five chamber bags as the storage container and Jungnickel medium without plant growth regulators or vitamins.
The wild species could be stored for 6 to 9 months.
After cryopreservation by PVS2 vitrification, recovery of the cultivars averaged 61% and of the wild species 30%. A calculation of the effort required to establish and maintain a safety duplication of the whole collection demonstrated that the application of in vitro cold storage is too labour intensive to be maintained.
The development of an effective method for cryopreservation is required for cost effective long-term storage.

I International Symposium on Cryopreservation in Horticultural Species

M. Höfer

cryopreservation, Fragaria, germplasm conservation, in vitro cold storage, PVS2

https://doi.org/10.17660/ActaHortic.2011.908.54