CRYOPRESERVATION OF EMBRYONIC AXES OF MELIA AZEDARACH L. AND CAMELLIA SINENSIS L. BY ENCAPSULATION-DEHYDRATION

Melia azedarach L., and Camellia sinensis L. were evaluated for long-term storage in liquid nitrogen (LN). Cryopreservation is an appropriate method for long-term conservation of plant genetic resources.
Encapsulation was tested with embryonic axes of M. azedarach and C. sinensis which were encapsulated in MS medium supplemented with 3% (w/v) Na-alginate, 100 mM CaCl₂ and 0.75 M sucrose and desiccated for 1 h.
The moisture content of embryonic axes of both species after treatment with sucrose and desiccation was around 18-20%. Embryonic axes were plunged in to LN and held for at least 1 h.
Following cryopreservation, embryonic axes were cultured on solid MS medium.
For M. azedarach no survival after freezing was observed for non-pretreated and non-encapsulated embryonic axes.
Similarly after freezing no survival was observed for pretreated and non-encapsulated embryonic axes, but 42% of embryogenic axis survived after pretreated and encapsulation.
For C. sinensis, non-encapsulated embryonic axes pretreated with sucrose and after dehydration did not survive.
However all pretreated and encapsulated embryonic axes withstood freezing in LN.

I International Symposium on Cryopreservation in Horticultural Species

B. Kaviani

embryonic axes, cryopreservation, encapsulation-dehydration, Melia azedarach L., Camellia sinensis L.

https://doi.org/10.17660/ActaHortic.2011.908.31