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Articles

CRYOPRESERVATION OF IRIS PUMILA SHOOT TIPS BY VITRIFICATION

Article number

908_47

Pages

355 – 359

Language

English

Abstract

A protocol for cryopreservation of Iris pumila shoot tips using a PVS2- vitrification procedure was developed.
Shoot tips, excised from in vitro grown shoots, were acclimated at 4°C for two days before vitrification procedure.
Following this preculture, shoot tips were treated with a loading solution for 30 min at 25°C, PVS2 solution for different times (from 5 to 40 min) at 0°C and then directly plunged into liquid nitrogen for at least one day.
Re-warming was performed in water bath at 40°C for 1 min.
After re-warming, the PVS2 was drained and replaced with unloading solution containing 1.2 M sucrose for 20 min.
Washed explants were cultured on medium for shoot development and multiplication.
The best results were obtained after PVS2 treatment for 20 min before immersion in liquid nitrogen.
Shoot tips regrowth was observed within 3 weeks and new shoots were regenerated and multiplied (55%) without calli phase within 12 weeks.

Publication

I International Symposium on Cryopreservation in Horticultural Species

Authors

S. Jevremović, A. Subotić, C. Benelli, A. De Carlo, M. Lambardi

Keywords

dwarf iris, PVS2 solution, in vitro proliferation

Full text

https://doi.org/10.17660/ActaHortic.2011.908.47

Online Articles (65)

1. GLASS TRANSITION DETERMINATION IN ALLIUM SHOOT TIPS AFTER DEHYDRATION

J. Zamecnik | M. Faltus | R. Kotkova | V. Hejnak

2. THERMAL ANALYSES BY DIFFERENTIAL SCANNING CALORIMETRY FOR CRYOPRESERVATION OF POTATO SHOOT TIPS

A. Kaczmarczyk | C. Zanke | A. Senula | M. Grübe | E.R.J. Keller

3. THERMAL EVENTS IN CALCIUM ALGINATE BEADS DURING ENCAPSULATION DEHYDRATION AND ENCAPSULATION-VITRIFICATION PROTOCOLS

R. Gámez-Pastrana | M.T. González-Arnao | Y. Martínez-Ocampo | F. Engelmann

4. GENE EXPRESSION IN RESPONSE TO CRYOPROTECTANT AND LIQUID NITROGEN EXPOSURE IN ARABIDOPSIS SHOOT TIPS

G.M. Volk | A. Henk | C. Basu

5. CRYOPRESERVATION AND PROTEOMIC ANALYSIS OF VANILLA (V. PLANIFOLIA A.) APICES TREATED WITH OSMOPROTECTANTS

6. ULTRASTRUCTURAL CHANGES IN SUSPENSION CULTURES OF BANANA (MUSA SPP. AAA) DURING CRYOPRESERVATION BY VITRIFICATION

C.-X. Xu | Y.-N. Li | X.-F. Deng | H.-B. Chen | B. Panis

7. TOPOGRAPHY DETERMINES THE IMPACT OF REACTIVE OXYGEN SPECIES ON SHOOT APICAL MERISTEMS OF RECALCITRANT EMBRYOS OF TROPICAL SPECIES DURING PROCESSING FOR CRYOPRESERVATION

8. EFFECT OF DMSO ON CASTOR BEAN POLLEN GRAIN VIABILITY

9. EFFECT OF GLYCEROL AS A CRYOPROTECTANT FOR CASTOR BEAN POLLEN FOR DIFFERENT STORAGE PERIODS

10. THE EFFECT OF ANTIOXIDANTS ON THE CRYOPRESERVATION RECOVERY OF TWO POTATO CULTIVARS FOLLOWING POST-THAWING CULTURE

P. Canepa | A. Panta | D. Tay

11. DESICCATION STUDIES OF DORMANT BUDS FROM SELECTED WOODY HORTICULTURAL PLANT SPECIES

M.M. Jenderek | D. Ellis | J. Postman | E. Stover

12. LONG-TERM SURVIVAL OF CRYOPRESERVED GERMPLASM: CONTRIBUTING FACTORS AND ASSESSMENTS FROM THIRTY YEAR OLD EXPERIMENTS

13. IS THE IN VITRO ESTABLISHMENT A CRITICAL POINT IN THE EPIGENETIC STABILITY OF THE CRYOPRESERVED HOPS (HUMULUS LUPULUS L.)?

E.L. Peredo | E. Cires | J.A. Fernández Prieto | M.A. Revilla | R. Arroyo-García

14. CRYOBANKING OF BANANA (MUSA SP.) GERMPLASM IN INDIA: EVALUATION OF AGRONOMIC AND MOLECULAR TRAITS OF CRYOPRESERVED PLANTS

15. NUCLEAR DNA INTEGRITY OF CRYOPRESERVED EMBRYONIC AXES OF ANADENANTHERA COLUBRINE (VELL.) BRENAN

16. ENCAPSULATION-DEHYDRATION METHOD ELEVATES EMBRYOGENIC ABILITIES OF GENTIANA KURROO CELL SUSPENSION AND CARRYING ON GENETIC STABILITY OF ITS REGENERANTS AFTER CRYOPRESERVATION

A. Mikuła | K. Tomiczak | A. Wójcik | J.J. Rybczyński

17. DROPLET VITRIFICATION: THE FIRST GENERIC CRYOPRESERVATION PROTOCOL FOR ORGANIZED PLANT TISSUES?

B. Panis | B. Piette | E. André | I. Van den houwe | R. Swennen

18. ENCAPSULATION-DEHYDRATION FOR CRYOPRESERVATION: PAST, PRESENT AND FUTURE

F. Engelmann

19. APPLICATION OF ALTERNATIVE LOADING SOLUTIONS TO GARLIC AND CHRYSANTHEMUM IN DROPLET-VITRIFICATION PROCEDURES

20. DEVELOPMENT OF ALTERNATIVE PLANT VITRIFICATION SOLUTIONS TO BE USED IN DROPLET-VITRIFICATION PROCEDURES

21. CRYOPRESERVATION OF FRUIT TREE SPECIES THROUGH ENCAPSULATION-DEHYDRATION AT THE CRA – FRUIT RESEARCH CENTRE OF ROME

22. CRYOPRESERVATION OF ANCIENT APPLE CULTIVARS OF VENETO: A COMPARISON BETWEEN PVS2-VITRIFICATION AND DORMANT-BUD TECHNIQUES

23. CRYOPRESERVATION OF BYRSONIMA INTERMEDIA A. JUSS. EMBRYOS USING DIFFERENT MOISTURE CONTENTS

24. EFFECT OF CRYOPRESERVATION ON GROWTH OF DIFFERENT ELITE EMBRYOGENIC CELL LINES OF NORWAY SPRUCE (PICEA ABIES (L.) KARST.)

B. Dedicova | O. Nilsson | U. Egertsdotter

25. CRYOPRESERVATION OF VANDA COERULEA PROTOCORM-LIKE BODIES BY DROPLET-VITRIFICATION

N. Jitsopakul | K. Thammasiri | K. Ishikawa

26. CRYOPRESERVATION OF AVOCADO EMBRYOGENIC CULTURES

E. Guzmán | F. Bradai | B. Panis | C. Sánchez-Romero

27. CRYOPRESERVATION OF DATE PALM HIGHLY REGENERABLE TISSUES USING VITRIFICATION PROCEDURES

28. CRYOPRESERVATION OF ANADENANTHERA COLUBRINE (VELL.) BRENAN EMBRYONIC AXES

29. CRYOPRESERVATION OF EMBRYONIC AXES OF CASTOR BEAN CULTIVAR ‘AL-GUARANY 2002’

30. CRYOPRESERVATION BY VITRIFICATION OF EMBRYOGENIC CALLUS OF WILD CROCUS (CROCUS HYEMALIS AND CROCUS MOABITICUS)

31. CRYOPRESERVATION OF EMBRYONIC AXES OF MELIA AZEDARACH L. AND CAMELLIA SINENSIS L. BY ENCAPSULATION-DEHYDRATION

B. Kaviani

32. CRYOPRESERVATION OF LILY SEEDS BY ENCAPSULATION-DEHYDRATION

B. Kaviani

33. TOMATO POLLEN STORAGE AT FREEZE AND CRYOGENIC TEMPERATURE – EFFECTS ON VIABILITY AND FECUNDITY

CH. Karipidis | D. Douma

34. POLLEN CRYOPRESERVATION OF CAMELLIA

B. Li | H. Wang | Y. Liu

35. A PRELIMINARY STUDY OF THE DROPLET VITRIFICATION METHOD APPLIED ON LILAC

A. Nukari | J. Laamanen | M. Uosukainen

36. IN VITRO PROPAGATION OF BLACKBERRY AND RASPBERRY AFTER COLD STORAGE OF ENCAPSULATED SHOOT TIPS

DJ. Ružić | T. Vujović | R. Cerović

37. CRYOPRESERVATION OF SHOOT TIPS OF BACOPA MONNIERI (L.) WETTST. BY VITRIFICATION TECHNIQUE

N. Sharma | R. Satsangi | R. Pandey

38. TWO-STEP PRECONDITIONING – A FEASIBLE METHOD FOR CRYOPRESERVATION OF FORTUNELLA POLYANDRA SHOOT TIPS USING VITRIFICATION TECHNIQUE

D.C. Chandrabalan | M.M. Clyde | M.N. Normah

39. LONG-TERM STORAGE OF FRASER PHOTINIA SHOOT TIPS VIA CONVENTIONAL AND DROPLET VITRIFICATION TECHNIQUES

H. Akdemir | Y. Ozden Tokatli

40. CRYOPRESERVATION BY ENCAPSULATION-DEHYDRATION OF IN VITRO GROWN SHOOT BUDS OF ROSA ‘NEW DAWN’

B. Pawlowska | A. Bach

41. CRYOPRESERVATION OF IN VITRO-GROWN SHOOT TIPS OF MORUS SPP. AND PYRUS COSSONII REHDER BY ENCAPSULATION-DEHYDRATION

S. Gupta

42. IN VITRO PROPAGATION AND CRYOPRESERVATION OF STEVIA REBAUDIANA USING A VITRIFICATION METHOD

M. Shatnawi | H. Al-Dmoor | M. Ateyat | S. Abubaker | R.A. Shibli

43. A COMPARISON OF VITRIFICATION AND DROPLET VITRIFICATION PROCEDURES FOR THE CRYOPRESERVATION OF IN VITRO GROWN BLACK CHOKEBERRY SHOOT TIPS

D. Tanaka | A. Nishiuchi | T. Niino | T. Matsumoto

44. PREPARATION OF SHOOT TIPS BY SUCROSE AND DEHYDRATION PRE-TREATMENT OF ULLUCUS TUBEROSUS CAL. FOR CRYOPRESERVATION

J. Zamecnikova | E. Fernandez | I. Viehmannova | J. Zamecnik | M. Faltus

45. CRYOPRESERVATION OF ULLUCO (ULLUCUS TUBEROSUS CAL.) AND OCA (OXALIS TUBEROSA MOL.) SHOOT TIPS USING THE PVS2 DROPLET-VITRIFICATION METHOD

D.F. Sánchez | A. Panta | D. Tay | W. Roca

46. CRYOPRESERVATION OF ILEX DUMOSA (AQUIFOLIACEAE) GERMPLASM

N.R. Dolce | H.Y. Rey | L.A. Mroginski

47. CRYOPRESERVATION OF IRIS PUMILA SHOOT TIPS BY VITRIFICATION

S. Jevremović | A. Subotić | C. Benelli | A. De Carlo | M. Lambardi

48. CHOOSING AND APPLYING CRYOPRESERVATION PROTOCOLS TO NEW PLANT SPECIES OR TISSUES

B.M. Reed

49. CRYOTHERAPY OF SHOOT TIPS: A NEWLY EMERGING TECHNIQUE FOR EFFICIENT ELIMINATION OF PLANT PATHOGENS

50. ATTEMPTS TO ERADICATE TWO PELARGONIUM VIRUSES (PFBV AND PLPV) BY MERISTEM CULTURE AND SHOOT-TIP CRYOTHERAPY

51. STUDY OF SLOW GROWTH CONDITIONS OF RANUNCULUS IN VITRO SHOOTS

M. Beruto | S. Rinino | A. Bisignano | M. Fibiani

52. CONSERVATION OF POTATO GERMPLASM IN THE CZECH REPUBLIC

M. Faltus | J. Zamecnik | J. Domkarova | L. Kreuz | V. Horackova

53. THE CURRENT CONSERVATION STATUS OF HORTICULTURAL GENETIC RESOURCES AND THEIR CRYOPRESERVATION FUTURE IN SYRIA

S. Abdullateef | I. Pinker | M. Böhme

54. CONSERVATION STRATEGY OF GENETIC RESOURCES FOR STRAWBERRY IN GERMANY

M. Höfer

55. CRYOPRESERVATION OF GARLIC FOR THE ESTABLISHMENT OF A EUROPEAN CORE COLLECTION

C. Zanke | J. Zamecnik | T. Kotlińska | M. Olas | E.R.J. Keller

56. CRYOBANKING CASSAVA GERMPLASM AT IITA

D. Dumet | S. Korie | A. Adeyemi

57. CURRENT DEVELOPMENT AND APPLICATION OF PLANT CRYOPRESERVATION IN LATIN AMERICA AND THE CARIBBEAN

M.T. González-Arnao | F. Engelmann

58. PROGRESS IN THE CZECH HOP GERMPLASM CRYOCONSERVATION

M. Faltus | J. Zamecnik | P. Svoboda | J. Patzak | V. Nesvadba

59. CRYOPRESERVATION AS A TOOL FOR THE MANAGEMENT OF COCONUT GERMPLASM

60. CRYOPRESERVATION OF MINT – ROUTINE APPLICATION IN A GENEBANK, EXPERIENCE AND PROBLEMS

A. Senula | E.R.J. Keller

61. CRYOPRESERVATION OF DORMANT BUDS FROM TEMPERATE FRUIT CROPS TO OPTIMISE WORKING COLLECTION RESOURCES

C. Vogiatzi | B.W.W. Grout | T.B. Toldam-Andersen | J. Green

62. CRYOPRESERVATION OF MULBERRY WINTER BUDS IN JAPAN

K. Fukui | K. Shirata | T. Niino | I.M. Kashif

63. CRYOPRESERVATION OF AN AZALEA GERMPLASM COLLECTION

J. Van Huylenbroeck | E. Calsyn

64. ALLIACEAE IN CRYOPRESERVATION, ACHIEVEMENTS AND CONSTRAINTS

E.R.J. Keller | A. Senula | C. Zanke

65. THE EFFECT OF PRE-CULTURE TEMPERATURE TREATMENT ON THE CRYOPRESERVATION OF POTATO SHOOT-TIPS

A. Panta | D. Tay | C. Ynouye | W. Roca