CRYOPRESERVATION OF EMBRYONIC AXES OF CASTOR BEAN CULTIVAR ‘AL-GUARANY 2002’

The castor bean (Ricinus communis) is an oleaginous plant with many applications in the society and economy.
Castor bean seeds tolerate storage in seed banks for a short period compared to orthodox seeds, probably due to its high lipid content.
This study aimed elaborate a cryopreservation protocol for castor bean embryonic axes.
After seeds disinfection, embryonic axes of the cultivar ‘AL-Guarany 2002’ were isolated, dehydrated and inoculated directly on culture medium (control) or cryostored in liquid nitrogen at -196°C, during one hour, in the presence or absence of cryoprotectant solutions (PVS2 or modified PVS2) prior to inoculation on culture.
It was obtained 100% of in vitro regeneration for all treatments.
After acclimatization a surviving rate of 100% was obtained for embryonic axes cryostored only in liquid nitrogen.
Axes stored in the presence of PVS2 presented a survival rate of 35%. The results obtained showed that the cryopreservation of castor bean embryonic axes in LN without cryoprotectants is a suitable technique to preserve the genetics resources of this species.

I International Symposium on Cryopreservation in Horticultural Species

G.F. Nogueira, R. Paiva, A.C.A.L. Campos, F.C. Nery, D.P. Vargas, P.D. de O. Paiva

Ricinus communis, cryoprotectant, micropropagation, PVS2, conservation

https://doi.org/10.17660/ActaHortic.2011.908.29