CRYOPRESERVATION OF SHOOT TIPS OF BACOPA MONNIERI (L.) WETTST. BY VITRIFICATION TECHNIQUE

A protocol for cryopreservation of in vitro shoot tips of Bacopa monnieri (L.) Wettst., a medicinal plant of high commercial value was developed.
Shoot tips (about 1 mm in length) excised from 4- to 24-week-old proliferating shoot cultures were precultured on MS medium supplemented with various cryoprotectants before dehydration with PVS2 at 0°C. The dehydrated shoot tips were directly immersed in liquid N₂. Following cryopreservation, and after rapid rewarming at 45°C, shoot tips were quickly washed with 1.2 M sucrose solution and then plated on solidified shoot culture medium.
Shoot tips were successfully cryopreserved by vitrification, when they were precultured on medium supplemented with 5% DMSO for two days before dehydration.
Preculture of shoot tips with sucrose at 25°C significantly improved the survival and shoot regeneration rate of cryopreserved shoot tips.
Regenerated plantlets were successfully transferred to pots.

I International Symposium on Cryopreservation in Horticultural Species

N. Sharma, R. Satsangi, R. Pandey

Bacopa monnieri, Brahmi, cryopreservation, medicinal plant, memory vitalizer, vitrification, PVS2

https://doi.org/10.17660/ActaHortic.2011.908.37