Articles
CRYOPRESERVATION OF AVOCADO EMBRYOGENIC CULTURES
Article number
908_26
Pages
215 – 218
Language
English
Abstract
The establishment of an efficient cryopreservation protocol for avocado embryogenic cultures is considered of outermost importance in order to avoid problems related to long-term in vitro maintenance such as risk of contamination, somaclonal variation or loss of embryogenic competence.
Three cryopreservation protocols were compared: a classical vitrification-based protocol, an ultra-fast freezing method using droplet vitrification on aluminium foil strips and a slow freezing method (-1°C/min). Post-thaw survival (i.e explants resuming growth 5 weeks after thawing). was genotype-dependent, but in general, higher frequencies of regrowth were obtained when using vitrification-based protocols.
The type of embryogenic tissue used as cryopreservation explants had a significant influence on survival.
When using embryogenic callus or somatic embryos at their early developmental stage all explants resumed growth five weeks after thawing.
Three cryopreservation protocols were compared: a classical vitrification-based protocol, an ultra-fast freezing method using droplet vitrification on aluminium foil strips and a slow freezing method (-1°C/min). Post-thaw survival (i.e explants resuming growth 5 weeks after thawing). was genotype-dependent, but in general, higher frequencies of regrowth were obtained when using vitrification-based protocols.
The type of embryogenic tissue used as cryopreservation explants had a significant influence on survival.
When using embryogenic callus or somatic embryos at their early developmental stage all explants resumed growth five weeks after thawing.
Authors
E. Guzmán, F. Bradai, B. Panis, C. Sánchez-Romero
Keywords
droplet vitrification, embryogenic tissue, Persea americana, vitrification-based protocols, slow freezing
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