Articles
TOMATO POLLEN STORAGE AT FREEZE AND CRYOGENIC TEMPERATURE – EFFECTS ON VIABILITY AND FECUNDITY
Article number
908_33
Pages
257 – 263
Language
English
Abstract
Storage at cryogenic temperatures is considered a successful method of retaining pollen viability in many plant species.
Storage at 20°C has also been reported as a convenient method of pollen preservation with satisfactory results.
In this study, the effects of two storage methods on tomato pollen viability and fecundity have been compared.
Tomato pollen was stored at 20°C using CaCl2 as desiccant and at 196°C after modification of its moisture content to 6.5-7% (wet weight basis). After 6, 8, 10, 12 and 18 months, in vitro pollen germination and pollen tube growth were tested following rehydration for 3 h at 100% R.H. and 15°C, and incubation for 6 h on semisolid substrate (1% agar, 12% sucrose and 50 mg L-1 H3BO3). Respiration activity was measured in pollen stored at both 20 and 196°C for 6 and 12 months in liquid substrate (12% sucrose and 50 mg L-1 H3BO3). Fecundity of stored pollen was assessed by artificial pollinations conducted at the same time.
Pollen stored at 20°C retained high in vitro germination for 10 months.
However, after only 6 months at 20°C, pollen tube length and respiration activity declined with a negative impact on pollen fecundity, as indicated by the reduced number of seeds per fruit in fruits obtained by artificial pollination.
Pollen germination ability significantly declined after 12 months.
On the other hand, pollen stored at 196°C for 18 months exhibited similar in vitro germination, pollen tube growth and respiration activity to those recorded for fresh pollen.
Furthermore, artificial pollination with cryogenically stored pollen resulted in similar fruit set and number of seeds per fruit to those observed after artificial pollination with fresh pollen.
Storage at 20°C has also been reported as a convenient method of pollen preservation with satisfactory results.
In this study, the effects of two storage methods on tomato pollen viability and fecundity have been compared.
Tomato pollen was stored at 20°C using CaCl2 as desiccant and at 196°C after modification of its moisture content to 6.5-7% (wet weight basis). After 6, 8, 10, 12 and 18 months, in vitro pollen germination and pollen tube growth were tested following rehydration for 3 h at 100% R.H. and 15°C, and incubation for 6 h on semisolid substrate (1% agar, 12% sucrose and 50 mg L-1 H3BO3). Respiration activity was measured in pollen stored at both 20 and 196°C for 6 and 12 months in liquid substrate (12% sucrose and 50 mg L-1 H3BO3). Fecundity of stored pollen was assessed by artificial pollinations conducted at the same time.
Pollen stored at 20°C retained high in vitro germination for 10 months.
However, after only 6 months at 20°C, pollen tube length and respiration activity declined with a negative impact on pollen fecundity, as indicated by the reduced number of seeds per fruit in fruits obtained by artificial pollination.
Pollen germination ability significantly declined after 12 months.
On the other hand, pollen stored at 196°C for 18 months exhibited similar in vitro germination, pollen tube growth and respiration activity to those recorded for fresh pollen.
Furthermore, artificial pollination with cryogenically stored pollen resulted in similar fruit set and number of seeds per fruit to those observed after artificial pollination with fresh pollen.
Authors
CH. Karipidis, D. Douma
Keywords
Lycopersicon esculentum, pollen storage, cryopreservation
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