Articles
ULTRASTRUCTURAL CHANGES IN SUSPENSION CULTURES OF BANANA (MUSA SPP. AAA) DURING CRYOPRESERVATION BY VITRIFICATION
Article number
908_6
Pages
73 – 81
Language
English
Abstract
Using transmission electron microscopy, ultrastructural changes taking place during the application of a vitrification protocol of embryogenic cells of Beida Aijiao were studied.
The results showed that the control cells contained a lot of organelles, a regular nucleolus envelope and intact plasma membranes.
After treatment with 25% PVS2, some changes could be observed, such as smaller vacuoles, more phenolic compounds, swollen organelles and appearance of many lipid bodies.
Also multi- membranous structures were observed and many vesicles appeared between the plasma membrane and the cell wall.
Moreover, the phenomenon of plasmolysis remained limited.
Only after dehydration with 100% PVS2 did plasmolysis became more severe.
Nucleus envelopes in these cells were malformed.
Though the cytoplasm and the nucleus became more electricity-dense and a lot of heterochromatin appeared, the plasma membrane was still intact.
The ultrastructure of cells after freezing, thawing and unloading was similar to that of those cells after dehydration.
After 2 weeks of post-thaw recovery, the ultrastructure of survival cells was similar to that of the control cells.
The results showed that the control cells contained a lot of organelles, a regular nucleolus envelope and intact plasma membranes.
After treatment with 25% PVS2, some changes could be observed, such as smaller vacuoles, more phenolic compounds, swollen organelles and appearance of many lipid bodies.
Also multi- membranous structures were observed and many vesicles appeared between the plasma membrane and the cell wall.
Moreover, the phenomenon of plasmolysis remained limited.
Only after dehydration with 100% PVS2 did plasmolysis became more severe.
Nucleus envelopes in these cells were malformed.
Though the cytoplasm and the nucleus became more electricity-dense and a lot of heterochromatin appeared, the plasma membrane was still intact.
The ultrastructure of cells after freezing, thawing and unloading was similar to that of those cells after dehydration.
After 2 weeks of post-thaw recovery, the ultrastructure of survival cells was similar to that of the control cells.
Authors
C.-X. Xu, Y.-N. Li, X.-F. Deng, H.-B. Chen, B. Panis
Keywords
banana, embryogenic cell suspension, cryopreservation by vitrification, ultrastructural changes
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