Articles
PROGRESS IN THE CZECH HOP GERMPLASM CRYOCONSERVATION
Article number
908_58
Pages
453 – 460
Language
English
Abstract
In this study the applicability of a new cryopreservation method for conservation of hop germplasm was evaluated.
Thirty-five cultivars of Humulus lupulus and one clone of Humulus japonicas were introduced into in vitro conditions, evaluated on viral infection, cryopreserved and recovered for tests of genotype stability.
Altogether 72 independent experiments were performed for verification of the cryopreservation method.
The average explant post-thaw recovery of all evaluated accessions was 38% whereas 72% accessions had post-thaw recovery higher than 30%. The results guaranteed successful recovery of all cryopreserved cultivars.
Tests of genotype stability by SSR and STS analyses did not detect any changes due to cryopreservation.
Thirty-five cultivars of Humulus lupulus and one clone of Humulus japonicas were introduced into in vitro conditions, evaluated on viral infection, cryopreserved and recovered for tests of genotype stability.
Altogether 72 independent experiments were performed for verification of the cryopreservation method.
The average explant post-thaw recovery of all evaluated accessions was 38% whereas 72% accessions had post-thaw recovery higher than 30%. The results guaranteed successful recovery of all cryopreserved cultivars.
Tests of genotype stability by SSR and STS analyses did not detect any changes due to cryopreservation.
Authors
M. Faltus, J. Zamecnik, P. Svoboda, J. Patzak, V. Nesvadba
Keywords
cryopreservation, Humulus lupulus L., liquid nitrogen
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