Articles
CRYOPRESERVATION OF MULBERRY WINTER BUDS IN JAPAN
Article number
908_62
Pages
483 – 488
Language
English
Abstract
The National Institute of Agrobiological Sciences (NIAS) genebank contains 1,470 accessions of Morus species (mulberry plants) grown in a field genebank.
Among them, 1,236 accessions originated in temperate areas and were suitable for preservation by application of the dormant bud cryopreservation technique.
Starting in 2003 and ending in 2008, all 1,236 accessions were subjected to cryopreservation storage in liquid nitrogen (LN) tanks (vapour phase, -160°C). For each accession, 160 buds were packed in 8 cryotubes and stored according to the protocol developed for mulberry winter buds.
For the practical application of this cryostorage technique, it is very important to confirm that cryopreserved shoot tips maintain their viability during long-term storage.
This study examines the survival frequency of winter buds of Kenmochi mulberry that had been stored for 11.5 years in LN tanks and observed 98% survival.
For each year from 2003 to 2008, the post-storage survival/regrowth of 4 randomly selected accessions from all those placed into storage that year were assessed.
The survival frequencies for the 24 accessions tested ranged from 75 to 100%. These results confirm that the technique of dormant bud preservation developed for mulberry is appropriate and that mulberry plants can be safely preserved long-term in LN tanks.
Among them, 1,236 accessions originated in temperate areas and were suitable for preservation by application of the dormant bud cryopreservation technique.
Starting in 2003 and ending in 2008, all 1,236 accessions were subjected to cryopreservation storage in liquid nitrogen (LN) tanks (vapour phase, -160°C). For each accession, 160 buds were packed in 8 cryotubes and stored according to the protocol developed for mulberry winter buds.
For the practical application of this cryostorage technique, it is very important to confirm that cryopreserved shoot tips maintain their viability during long-term storage.
This study examines the survival frequency of winter buds of Kenmochi mulberry that had been stored for 11.5 years in LN tanks and observed 98% survival.
For each year from 2003 to 2008, the post-storage survival/regrowth of 4 randomly selected accessions from all those placed into storage that year were assessed.
The survival frequencies for the 24 accessions tested ranged from 75 to 100%. These results confirm that the technique of dormant bud preservation developed for mulberry is appropriate and that mulberry plants can be safely preserved long-term in LN tanks.
Authors
K. Fukui, K. Shirata, T. Niino, I.M. Kashif
Keywords
genebank, dormant buds, liquid nitrogen, long-term storage, practical conservation
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