Articles
CRYOPRESERVATION OF ANADENANTHERA COLUBRINE (VELL.) BRENAN EMBRYONIC AXES
Article number
908_28
Pages
227 – 231
Language
English
Abstract
The ex situ preservation and rational use of Anadenanthera colubrine demand conclusive information related to seed storage.
This study aimed determines a protocol for cryopreserving embryonic axes of A. colubrine seeds.
Embryonic axes were treated with three concentrations (5, 10, and 15%) of DMSO and glycerol and maintained in liquid nitrogen (-196°C), ultra freezer (-80°C) and freezer (-20°C) for 15, 30, and 150 days.
Moisture content of the embryonic axes was determined before and after each storage period.
Embryonic axes immersed in chemical cryoprotectants and maintained at -196, -80, and -20°C, absorbed the solutions and had moisture contents higher than 65%. Under these conditions, no germination was observed.
Embryonic axes that were stored in the absence of cryoprotectants presented viability around 100%, independently of the cryostorage period.
This study aimed determines a protocol for cryopreserving embryonic axes of A. colubrine seeds.
Embryonic axes were treated with three concentrations (5, 10, and 15%) of DMSO and glycerol and maintained in liquid nitrogen (-196°C), ultra freezer (-80°C) and freezer (-20°C) for 15, 30, and 150 days.
Moisture content of the embryonic axes was determined before and after each storage period.
Embryonic axes immersed in chemical cryoprotectants and maintained at -196, -80, and -20°C, absorbed the solutions and had moisture contents higher than 65%. Under these conditions, no germination was observed.
Embryonic axes that were stored in the absence of cryoprotectants presented viability around 100%, independently of the cryostorage period.
Authors
F.C. Nery, R. Paiva, A.C.A.L. Campos, G.F. Nogueira, V.C. Stein, A.A. Alvarenga
Keywords
storage, seeds, forest species
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