Articles

Using the genetic diversity of plum to explore the complexity of fruit ripening

Article number
1194_188
Pages
1337 – 1344
Language
English
Abstract
Japanese plums, which include most of the fresh-market plums commercialized globally, represent the most abundant and variable group among tree species.
We characterized and compared two Japanese plum cultivars, ‘Santa Rosa’ (SR) and its bud-sport mutant ‘Sweet Miriam’ (SM). These cultivars share the same genetic background but display contrasting ripening behaviors (SR, climacteric and SM, non-climacteric). Both cultivars differ in their ripening-related changes, what influences properties related to fruit quality, including color, taste, texture and aroma.
The main objective of this research was to compare SR and SM in relation to ethylene levels as well as ripening-related changes during postharvest.
Fruits from each cultivar were harvested at the mature stage (flesh firmness of ~35 N which coincided with the preclimacteric stage in SR), stored at 20°C and 90% relative humidity and evaluated at harvest and after 7 and 14 days of storage.
Targeted gene expression and metabolite concentrations were assessed.
Our results show that ethylene biosynthesis-related genes had significantly higher transcript accumulation levels in SR than SM throughout postharvest.
Concerning ripening-related changes, flesh and skin color differences between cultivars were supported by contrasting anthocyanin and carotenoid biosynthesis-related gene expression.
Sugar composition was also altered between both cultivars (SR presented higher sucrose concentrations, though SM had higher sorbitol abundance); titratable acidity values were significantly lower in SM; while fruit softening rates as well as cell wall polysaccharide-related expression profiles were higher in SR as compared to SM. Overall, this experimental system could provide excellent opportunities for unraveling the complex process(es) associated with climacteric/non-climacteric fruit ripening.

Publication
Authors
M. Farcuh, P. Saha, E. Blumwald
Keywords
Prunus salicina, climacteric behavior, bud-sport mutant, fruit quality, ethylene
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