Articles
APPLICATION OF IN VITRO CULTURE TO ONION AND GARLIC FOR THE MANAGEMENT AND USE OF GENETIC RESOURCES AT GATERSLEBEN
Article number
433_13
Pages
141 – 150
Language
Abstract
The genebank Gatersleben and the Taxonomy department of the IPK maintain a large collection of Allium. In vitro culture is used for preservation of vegetatively propagated forms.
Sucrose-induced bulblets were stored at different temperatures.
Characterization of bulblet viability was performed by growth and ion efflux measurements.
Dormancy was compared in material stored at + 3°C with or without warm preculture at + 25°C and with or without treatments with ethylene.
Meristem culture was used for virus elimination.
Elimination rates depended on the clone and vary from 15 to 58%. Hybrids from distant crosses of onion with various wild species were obtained by embryo rescue via ovary culture.
They were verified by karyotype, isoenzyme, and RAPD analysis.
Sucrose-induced bulblets were stored at different temperatures.
Characterization of bulblet viability was performed by growth and ion efflux measurements.
Dormancy was compared in material stored at + 3°C with or without warm preculture at + 25°C and with or without treatments with ethylene.
Meristem culture was used for virus elimination.
Elimination rates depended on the clone and vary from 15 to 58%. Hybrids from distant crosses of onion with various wild species were obtained by embryo rescue via ovary culture.
They were verified by karyotype, isoenzyme, and RAPD analysis.
Publication
Authors
E.R. Joachim Keller, D. Lesemann
Keywords
Allium, ethylene treatment, hybridization, in vitro bulbs, meristem culture, slow growth storage, virus elimination
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