Articles
IMPROVEMENT OF GERMINATION OF VITEX AGNUS CASTUS L. SEEDS WITH SEED PRE-TREATMENTS
Article number
454_24
Pages
207 – 212
Language
Abstract
To investigate the germination ability of Vitex agnus castus L. seeds, pre-treatments with sulfuric acid (for 60–120 min), chilling (for 2 weeks at 5°C) and soaking in water or 400 ppm GA3 (for 6–10 hrs) were applied, followed by incubation at 20°C (for 2 weeks) and transfer to 30°C (for 1 week), 90% RH and natural light cycling.
Seeds incubated at 20°C or 30°C without any pretreatment failed to germinate.
Seeds scarified with sulfuric acid for 30 to 60 min followed by incubation at 20°C showed little germination.
However, upon transfer to 30°C, germination increased to 90–100%. Seeds pre-treated for 8–10 hrs with 400 ppm GA3 showed about 20% germination during incubation at 20°C. Upon transfer to 30°C, germination of chilled seeds increased to 60%, and of water and GA3-soaked seeds to about 80–90%. These results indicate that V. agnus castus L. seeds show seed coat and endosperm dormancy.
Seeds incubated at 20°C or 30°C without any pretreatment failed to germinate.
Seeds scarified with sulfuric acid for 30 to 60 min followed by incubation at 20°C showed little germination.
However, upon transfer to 30°C, germination increased to 90–100%. Seeds pre-treated for 8–10 hrs with 400 ppm GA3 showed about 20% germination during incubation at 20°C. Upon transfer to 30°C, germination of chilled seeds increased to 60%, and of water and GA3-soaked seeds to about 80–90%. These results indicate that V. agnus castus L. seeds show seed coat and endosperm dormancy.
Authors
S. Belhadj, D. Gerasopoulos, E. Maloupa
Keywords
Ornamental wild species, Mediterranean, Verbenaceae, propagation, germination, pre-treatment
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