Articles
SONICATION AS AN AID FOR HANDLING GERALDTON WAX (CHAMELAUCIUM UNCINATUM) POLLEN
Article number
454_43
Pages
365 – 372
Language
Abstract
Pollen in Chamelaucium species is exuded from the anthers in an oily substance and deposited onto the pollen presenter.
Pollen in oil was sonicated in 20% sucrose at 20 watts, then spun down at 8,000 rpm for 60 s.
Pollen germination was reduced by sonication (from 50% to 30%), and pollen tube length was reduced by about one third in 2 of the 3 lines tested.
When sonicated pollen was used on the stigma, good germination was observed; by 48 h there were as many pollen tubes at the base of the style as when using unsonicated pollen.
Overall 11.5% seed set was observed using sonicated pollen in intraspecific crosses of C. uncinatumm, and 7% in C. uncinatum x megalopetalum crosses.
Pollen in oil was sonicated in 20% sucrose at 20 watts, then spun down at 8,000 rpm for 60 s.
Pollen germination was reduced by sonication (from 50% to 30%), and pollen tube length was reduced by about one third in 2 of the 3 lines tested.
When sonicated pollen was used on the stigma, good germination was observed; by 48 h there were as many pollen tubes at the base of the style as when using unsonicated pollen.
Overall 11.5% seed set was observed using sonicated pollen in intraspecific crosses of C. uncinatumm, and 7% in C. uncinatum x megalopetalum crosses.
Authors
C. Newell, J. McComb, G. Lullfitz
Keywords
pollen germination, Chamelaucium megalopetalum, pollen tube growth
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