Articles
CONSTITUTIVE EXPRESSION OF β-GLUCURONIDASE GENE FUSED WITH STRESS-INDUCIBLE PROMOTER OF THE PATHOGENESIS-RELATED 1A PROTEIN GENE OF TOBACCO IN TRANSGENIC KALANCHOE BLOSSFELDIANA
Article number
454_44
Pages
373 – 376
Language
Abstract
Pathogenesis-related 1a (PR1a) promoter derived from PR1a protein gene of tobacco has been reported to conduct stress-induced or salicylic acid (SA) -induced expression in tobacco, potato, lettuce and torenia.
Constitutive expression, an abnormal response, of the promoter was reported only in an interspecific hybrid of Nicotiana glutinosa x Nicotiana debneyi. We report constitutive expression of ß-glucuronidase (GUS) gene fused with PR1a promoter in transgenic Kalanchoe blossfeldiana. The chimeric gene (PR1a/GUS) was introduced into K. blossfeldiana by Agrobacterium-mediated transformation.
The expression level of GUS gene among 45 individual transformants were analyzed.
The activity level in non-treated leaf of each plant was varied from near-zero to about 17000 nmoles 4 MU/mg protein/30min.
Thus, several plants showed very high activity even at healthy (not-induced) condition.
The activity level was almost same between non-treated and SA-treated leaf in most of transformants.
These results suggested that the stress-inducible promoter of tobacco did not conduct its nature function but showed abnormal response, constitutive expression, in K. blossfeldiana.
Constitutive expression, an abnormal response, of the promoter was reported only in an interspecific hybrid of Nicotiana glutinosa x Nicotiana debneyi. We report constitutive expression of ß-glucuronidase (GUS) gene fused with PR1a promoter in transgenic Kalanchoe blossfeldiana. The chimeric gene (PR1a/GUS) was introduced into K. blossfeldiana by Agrobacterium-mediated transformation.
The expression level of GUS gene among 45 individual transformants were analyzed.
The activity level in non-treated leaf of each plant was varied from near-zero to about 17000 nmoles 4 MU/mg protein/30min.
Thus, several plants showed very high activity even at healthy (not-induced) condition.
The activity level was almost same between non-treated and SA-treated leaf in most of transformants.
These results suggested that the stress-inducible promoter of tobacco did not conduct its nature function but showed abnormal response, constitutive expression, in K. blossfeldiana.
Authors
R. Aida, M. Shibata
Keywords
genetic transformation
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