Articles
INTROGRESSION IN JAPANESE EVERGREEN AZALEAS (RHODODENDRON KIUSIANUM AND R. KAEMPFERI)
Article number
454_37
Pages
325 – 328
Language
Abstract
Natural hybridization of Japanese evergreen azaleas has often generated morphological variations, which have been used for the parents of azalea breeding.
We investigated wild populations of evergreen azaleas in Kyushu composed of Rhododendron kiusianum, R. kaempferi and their interspecific hybrids.
Morphological characters such as leaf size and flower color clearly distinguished two species.
PCR-RFLP analysis of cpDNA could detect their specific bands in 16S rDNA region digested with HhaI restriction enzyme.
However, 24% of individuals, which were morphologically indistinguishable from R. kiusianum, possessed the cpDNA of R. kaempferi. This suggests that past introgression into R. kiusianum might have occurred.
We investigated wild populations of evergreen azaleas in Kyushu composed of Rhododendron kiusianum, R. kaempferi and their interspecific hybrids.
Morphological characters such as leaf size and flower color clearly distinguished two species.
PCR-RFLP analysis of cpDNA could detect their specific bands in 16S rDNA region digested with HhaI restriction enzyme.
However, 24% of individuals, which were morphologically indistinguishable from R. kiusianum, possessed the cpDNA of R. kaempferi. This suggests that past introgression into R. kiusianum might have occurred.
Authors
N. Kobayashi, S. Kawashima, T. Handa, K. Takayanagi, K. Arisumi
Keywords
chloroplast DNA, rDNA, PCR, RFLP
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