Articles
INFLUENCE OF AGAR BRANDS AND CONCENTRATION ON IN VITRO SHOOT MULTIPLICATION OF THE CHERRY ROOTSTOCK GISELA 5
Article number
468_26
Pages
209 – 218
Language
Abstract
Agar type can modify the reaction of a plant explant, independent of the other media components.
Agar types and concentrations were assessed with respect to good shoot multiplication without the occurrence of hyperhydricity in the culture of the sweet cherry rootstock Gisela 5 (Prunus cerasus x Prunus canescens). Shoot tips were cultured on Murashige and Skoog (1962) medium supplemented with (in mg l-1): BAP, 1; NAA, 0.1; GA3, 0.1, and sucrose 20 000. The basic medium was made with one of six brands of agar: Kalys 575(600); Kalys 500; Sigma; Difco-Bacto; Torlak; and a Local type used in the confectionery industry.
Three concentrations of each were used: 6, 6.5 and 7 g l-1. Optimum shoot multiplication without hyperhydric transformation was obtained on media containing 6, and especially 6.5 g l-1 of Kalys 575(600), Kalys 500 and Sigma agar.
On the media containing other agar types, the hyperhydricity symptoms were lessened, or completely disappeared (Sigma agar), only at the 7 g l-1 concentration.
Increasing agar levels reduced shoot growth with all types of agar.
Also, fresh and dry weights of cultures were reduced with increasing concentration of all agar types to 7 g l-1. Our studies showed that hyperhydricity, shoot number and length in Gisela 5 cultures were affected by the type and amount of gelling agents.
Agar types and concentrations were assessed with respect to good shoot multiplication without the occurrence of hyperhydricity in the culture of the sweet cherry rootstock Gisela 5 (Prunus cerasus x Prunus canescens). Shoot tips were cultured on Murashige and Skoog (1962) medium supplemented with (in mg l-1): BAP, 1; NAA, 0.1; GA3, 0.1, and sucrose 20 000. The basic medium was made with one of six brands of agar: Kalys 575(600); Kalys 500; Sigma; Difco-Bacto; Torlak; and a Local type used in the confectionery industry.
Three concentrations of each were used: 6, 6.5 and 7 g l-1. Optimum shoot multiplication without hyperhydric transformation was obtained on media containing 6, and especially 6.5 g l-1 of Kalys 575(600), Kalys 500 and Sigma agar.
On the media containing other agar types, the hyperhydricity symptoms were lessened, or completely disappeared (Sigma agar), only at the 7 g l-1 concentration.
Increasing agar levels reduced shoot growth with all types of agar.
Also, fresh and dry weights of cultures were reduced with increasing concentration of all agar types to 7 g l-1. Our studies showed that hyperhydricity, shoot number and length in Gisela 5 cultures were affected by the type and amount of gelling agents.
Publication
Authors
Dj. Ruzic, R. Cerovic
Keywords
Hyperhydricity, gelling agents, clonal propagation, Prunus hybrid rootstock
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