Articles
EXTRACTABILITY OF GIBBERELLIN-LIKE SUBSTANCES FROM TULIPS GROWN AT TWO TEMPERATURES IN EITHER THE LIGHT OR DARK
Article number
47_43
Pages
297 – 306
Language
Abstract
Tulipa gesneriana L. cv."Paul Richter" were rooted and cooled for 15 weeks at 9°C in the dark.
After an initial sampling, lots were transferred to either continuous light or dark conditions at either 9 or 17°C for one week.
Compared to dark-grown bulbs, there was a greater increase in shoot fresh weight in the light.
However, the scapes were longer in the dark.
Purification of the extracts by a double paper chromatography technique in combination with a dilution prior to GA bioassay by barely half-seeds, reduced the effect of co-extracted inhibitors.
The highest GA-activity was found in extracts from plants grown in the dark at 17°C. Light-grown plants yielded reduced activity.
The shoot yielded the greatest activity followed by scales and then roots.
Activity was greatest in the acidic free fraction with lesser amounts in the acidic bound and neutral free fractions.
After an initial sampling, lots were transferred to either continuous light or dark conditions at either 9 or 17°C for one week.
Compared to dark-grown bulbs, there was a greater increase in shoot fresh weight in the light.
However, the scapes were longer in the dark.
Purification of the extracts by a double paper chromatography technique in combination with a dilution prior to GA bioassay by barely half-seeds, reduced the effect of co-extracted inhibitors.
The highest GA-activity was found in extracts from plants grown in the dark at 17°C. Light-grown plants yielded reduced activity.
The shoot yielded the greatest activity followed by scales and then roots.
Activity was greatest in the acidic free fraction with lesser amounts in the acidic bound and neutral free fractions.
Publication
Authors
A. Alpi, A.A. De Hertogh
Keywords
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