Articles

PRELIMINARY EVALUATION OF DOUBLED HAPLOID-MATERIAL IN APPLE

Article number
538_102
Pages
587 – 592
Language
English
Abstract
At the Institute for Fruit Breeding in Dresden, anther and microspore culture and in situ parthenogenesis were used for successful haploid induction in apple.
At present, 107 lines of 9 different donor genotypes exist in vitro and 50 of them have already been transferred in situ. Determination of the ploidy level, by means of flow cytometry, demonstrated a distribution of diploid, triploid and tetraploid levels for the regenerated lines of one single donor genotype.
Using segregating isozyme loci as markers, 93% of the lines were found to be definitely homozygous.
Out of 30 lines of scab resistant donor genotypes, tested by PCR-analysis, 24 demonstrated the SCAR-Marker ALO7 linked to the Vf-gene for scab resistance from Malus floribunda.

Publication
Authors
M. Hoefer, C. Grafe
Keywords
haploid induction, homozygosity, Malus, ploidy level, scab resistance
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