Articles

EMBRYO CULTURE IN PRUNUS L. BREEDING

Article number
538_118
Pages
663 – 665
Language
Abstract
The use of early ripening genotypes in breeding programs and embryo rescue in vitro complex techniques had good results.
We have obtained a large early ripening hybrid population from the main early ripening cultivar crosses (‘Gronkavaya’, ‘Subarovskaya’, ‘Ranniaya Rozovaya’), population of interspesific hybrids of Prunus L. and F2 of the interspecific hybrids for further selection.
The best time of in vitro culture initiation was established.
Different methods of fruit and embryo sterilization were studied, the technique of hardened pits and soft pits sterilization was developped.
The system of embryo culture and medium composition for all stages of in vitro cultivation were elaborated.
We have proposed embryos dormancy breaking technique and the system of regenerant adaptation ex vitro. More than 12.000 embryos were cultivated.

Publication
Authors
N.V. Koukhartchik, S. Semenas
Keywords
Prunus, embryo, regeneration, dormancy, Belarus
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