Articles

GENOME INVESTIGATION IN SOUR CHERRY, P. CERASUS L.

Article number
538_66
Pages
375 – 379
Language
Abstract
Mitotic chromosome preparations of the sour cherry ‘Schattenmorelle’ were used to study the genome constitution of Prunus cerasus L. by genomic in situ hybridization (GISH) and karyotype analysis.
Total genomic DNA from the presumably donor species P. avium and P. fruticosa were used as the probes.
The probes were labelled with Digoxigenin-11-dUTP by nick translation.
The probe DNA’s of P. avium and of P. fruticosa each hybridized to 16 of the 32 chromosomes of sour cherry.
By means of the karyotype analysis of C-banded P. cerasus and P. avium chromosomes, it was possible to detect the P. avium chromosomes in the P. cerasus genome.
These results confirmed that P. avium and P. fruticosa are the donor species of the P. cerasus genome.

Publication
Authors
M. Schuster, H. Schreibner
Keywords
P. cerasus, P. avium, P. fruticosa, genomic in situ hybridization, karyotype,
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