Establishment of the cell suspension culture system for the jujube cultivar ‘Fengmiguan’

Suspension cell is an important tool for basic studies on plant biochemistry and molecular biology.
Jujube (Ziziphus jujuba), priced for its sweet and nutritious fruits, is the most economical fruit tree in the Rhamnaceae family.
To establish a cell suspension system, in vitro leaves of the jujube cultivar ‘Fengmiguan’ were used as explant.
Callus was induced on the medium MS+2.0 mg L^‑1 2,4-D+0.3 mg L^‑1 6-BA+0.5 mg L^‑1 TDZ. Two g callus were suspension cultured in 50 mL liquid medium.
Cells in medium MS+2.0 mg L^‑1 2,4-D+0.5 mg L^‑1 KT grew fastest in an ‘S’ curve, with a cycle of 21 d.
The establishment of jujube cell suspension provides a foundation for further gene function study.

V International Jujube Symposium

Y. Zhang, Q.Q. Yang, P. Chen, Q.C. Li, Z.S. Qiao, J.D. Li, J.C. Feng

jujube, cell suspension, medium, explants, in vitro culture

https://doi.org/10.17660/ActaHortic.2022.1350.29