Articles
DNA markers in hazelnut: a progress report
Article number
1379_10
Pages
61 – 72
Language
English
Abstract
European hazelnut, Corylus avellana L., is the major source of cultivars grown around the world.
DNA markers are useful for marker-assisted selection (MAS), especially in trees such as hazelnut with a long juvenile period.
This study provides an overview of PCR- and sequence-based DNA markers and their use in hazelnut.
Starting in the 1990s, random amplified polymorphic DNA (RAPD) markers were used for linkage mapping, and markers associated with self-incompatibility alleles and eastern filbert blight (EFB) resistance were identified.
Another dominant PCR-based marker, amplified polymorphic length polymorphism (AFLP), was also used to identify markers linked to EFB resistance.
High resolution melting (HRM), single characterized amplified region (SCAR), and single-strand conformation polymorphism (SSCP) markers were applied for fine mapping of the EFB resistance region in Jefferson. While RAPDs continue to be routinely used for MAS for EFB resistance, simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers have replaced them for other applications.
Over 900 SSR markers are available in hazelnut where they have been used for diversity assessment, cultivar identification, association mapping, core collection establishment, and quantitative trait loci (QTL) mapping.
SNPs from reduced representation sequencing were utilized in linkage mapping and to assess the diversity and domestication of the European hazelnut.
A consortium is developing a multi-species temperate tree nut array of 70,000 SNPs that dedicates at least 20,000 loci for hazelnut, a welcome addition to the toolkit.
The DNA marker resources are useful tools for assisting breeders in developing cultivars that meet the needs of growers and consumers alike.
DNA markers are useful for marker-assisted selection (MAS), especially in trees such as hazelnut with a long juvenile period.
This study provides an overview of PCR- and sequence-based DNA markers and their use in hazelnut.
Starting in the 1990s, random amplified polymorphic DNA (RAPD) markers were used for linkage mapping, and markers associated with self-incompatibility alleles and eastern filbert blight (EFB) resistance were identified.
Another dominant PCR-based marker, amplified polymorphic length polymorphism (AFLP), was also used to identify markers linked to EFB resistance.
High resolution melting (HRM), single characterized amplified region (SCAR), and single-strand conformation polymorphism (SSCP) markers were applied for fine mapping of the EFB resistance region in Jefferson. While RAPDs continue to be routinely used for MAS for EFB resistance, simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers have replaced them for other applications.
Over 900 SSR markers are available in hazelnut where they have been used for diversity assessment, cultivar identification, association mapping, core collection establishment, and quantitative trait loci (QTL) mapping.
SNPs from reduced representation sequencing were utilized in linkage mapping and to assess the diversity and domestication of the European hazelnut.
A consortium is developing a multi-species temperate tree nut array of 70,000 SNPs that dedicates at least 20,000 loci for hazelnut, a welcome addition to the toolkit.
The DNA marker resources are useful tools for assisting breeders in developing cultivars that meet the needs of growers and consumers alike.
Publication
Authors
N.V. Bassil, S.A. Mehlenbacher
Keywords
microsatellite marker, single nucleotide polymorphisms, germplasm, SSR, SNP, RAPD, AFLP, ISSR, GBS, ddRAD
Groups involved
Online Articles (78)