Articles
DETECTION OF PRUNUS NECROTIC RINGSPOT VIRUS BY BIOASSAY AND IEM TECHNIQUE DURING THE VEGETATION PERIOD OF SOUR CHERRY TREES
Article number
309_11
Pages
99 – 104
Language
English
Abstract
PNRSV was present in all four tested branches only in 11 out of 23 virus-infected sour cherry trees.
Twelve trees were selected for furhter experiments and virus detection was compared in frozen and freshly collected leaves every month from May till September.
The best time for taking samples from sour cherry trees to detect PNRSV was the early spring.
The freezing at minus 20 degrees Celsius of leaf samples decreased the virus detection and the number of virus particles in preparations, but the virus detection in frozen material stayed a the acceptable level till the end of testing period.
The defreezing of plant material reduced the virus infectivity but had no influence on its serological detecteion.
The IEM technique was more reliable than bioassay.
Twelve trees were selected for furhter experiments and virus detection was compared in frozen and freshly collected leaves every month from May till September.
The best time for taking samples from sour cherry trees to detect PNRSV was the early spring.
The freezing at minus 20 degrees Celsius of leaf samples decreased the virus detection and the number of virus particles in preparations, but the virus detection in frozen material stayed a the acceptable level till the end of testing period.
The defreezing of plant material reduced the virus infectivity but had no influence on its serological detecteion.
The IEM technique was more reliable than bioassay.
Authors
S. Kryczynski, M.S. Szyndel, A. Stawiszynska, A. Andersz-Wroblewska
Keywords
necrotic ringspot virus, bioassay, IEM, sour cherry
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