Articles
MONOCLONAL ANTIBODIES AGAINST PLUM POX VIRUS
Article number
309_24
Pages
169 – 174
Language
Abstract
Monoclonal antibodies (MAbs) were obtained to plum pox virus (PPV) after immunisation of BALB/c mice with purified PPV-W isolate.
Spleen cells from a mouse showing a high serum titre were used for fusion with Sp 2/0-Ag14 myeloma cells.
Culture supernatants were screened for specific antibody production against PPV-W using indirect ELISA. A total of 6 stable hybridoma lines were obtained producing MAb of IgG class.
One highly efficient antibody was chosen for practical purposes, and its applicability in PPV diagnostics has been studied since 1989 in comparison with polyclonal antibodies and accessible commercial kits.
The experiments have proven a comparable sensitivity with a detection limit ranging between 10 and 50 ng.
In routine diagnostics of plums our MAb either ranked higher as compared to the commercial kits (Boehringer, Bioreba) or gave similar results.
Spleen cells from a mouse showing a high serum titre were used for fusion with Sp 2/0-Ag14 myeloma cells.
Culture supernatants were screened for specific antibody production against PPV-W using indirect ELISA. A total of 6 stable hybridoma lines were obtained producing MAb of IgG class.
One highly efficient antibody was chosen for practical purposes, and its applicability in PPV diagnostics has been studied since 1989 in comparison with polyclonal antibodies and accessible commercial kits.
The experiments have proven a comparable sensitivity with a detection limit ranging between 10 and 50 ng.
In routine diagnostics of plums our MAb either ranked higher as compared to the commercial kits (Boehringer, Bioreba) or gave similar results.
Authors
M. Navrátil, D. Cikánek, I. Hilgert, H. Krištofová, R. Karešová
Keywords
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