Articles
PROPERTIES AND TRANSLATION STUDIES OF CHERRY LEAF ROLL VIRUS (CLRV)
Article number
309_4
Pages
45 – 54
Language
Abstract
The Mr of RNA 1 and RNA 2 of the walnut strain of cherry leaf roll virus (CLRV) were determined to be 2.31 × 106 (7.02 Kilobases, Kb) and 2.09 × 106 (6.33 Kb) respectively when assayed by electrophoresis under denaturing conditions.
Translation products of both RNAs in a rabbit reticulocyte cell-free system ranged from 30K to 195K. Anti-virion sera immunoprecipitated three major polypeptide products of 48K, 56K and 165K. The 56K band has the same size as the authentic coat protein as assayed by SDS-PAGE. Time course analysis reveals that the coat protein appears after five min whereas the 165K band corresponding to the polyprotein does so after 20 min.
Translation products of both RNAs in a rabbit reticulocyte cell-free system ranged from 30K to 195K. Anti-virion sera immunoprecipitated three major polypeptide products of 48K, 56K and 165K. The 56K band has the same size as the authentic coat protein as assayed by SDS-PAGE. Time course analysis reveals that the coat protein appears after five min whereas the 165K band corresponding to the polyprotein does so after 20 min.
Authors
V. Pallas, A. Rowhani, S. Daubert, G. Bruening
Keywords
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