Articles
STUDY ON PRUNUS NECROTIC RING SPOT VIRUS IN GREECE
Article number
309_6
Pages
63 – 72
Language
Abstract
Prunus necrotic ring spot virus (PNRSV) is one of the viruses occurring widespread in Prunus spp. in Greece.
The study of this virus was undertaken as part of a state research program aiming at the development of diagnostic techniques for certification purposes.
Surveys were undertaken in different parts of the country and PNRSV was found in all areas tested.
Ten different isolates from almond, peach, nectarine and plum trees were obtained and maintained in the indicator GF-305 seedlings.
These isolates varied in their pathogenicity, ranging from latent infection to severe bud failure and necrotic leaf spotting.
Different serological reagents were able to detect these isolates in DAS ELISA. Two commercial reagents and a laboratory one prepared from Dr Fulton’s PNRSV isolate G antiserum were assayed.
When these reagents were tried against purified virus of a severe Greek isolate from peach, their sensitivity in DAS ELISA was about 10 ng/ml virus.
The ELISA test sensitivity was increased at least two fold by the application of the cocktail ELISA technique.
When this technique was combined with an amplification of the enzyme reaction, 0.5 ng/ml virus were readily detected.
In view of preparation of local antiserum, purification of the virus was undertaken.
The molecular weight of the viral coat protein is the same as that cited in the bibliography (25 000 d).
The study of this virus was undertaken as part of a state research program aiming at the development of diagnostic techniques for certification purposes.
Surveys were undertaken in different parts of the country and PNRSV was found in all areas tested.
Ten different isolates from almond, peach, nectarine and plum trees were obtained and maintained in the indicator GF-305 seedlings.
These isolates varied in their pathogenicity, ranging from latent infection to severe bud failure and necrotic leaf spotting.
Different serological reagents were able to detect these isolates in DAS ELISA. Two commercial reagents and a laboratory one prepared from Dr Fulton’s PNRSV isolate G antiserum were assayed.
When these reagents were tried against purified virus of a severe Greek isolate from peach, their sensitivity in DAS ELISA was about 10 ng/ml virus.
The ELISA test sensitivity was increased at least two fold by the application of the cocktail ELISA technique.
When this technique was combined with an amplification of the enzyme reaction, 0.5 ng/ml virus were readily detected.
In view of preparation of local antiserum, purification of the virus was undertaken.
The molecular weight of the viral coat protein is the same as that cited in the bibliography (25 000 d).
Authors
C. Varveri
Keywords
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