GENOMIC ORGANIZATION OF APPLE CHLOROTIC LEAF SPOT CLOSTEROVIRUS (ACLSV)

A 7555-nucleotide long sequence (excluding the 3′ terminal poly A tail) of the P863 strain of apple chlorotic leaf spot closterovirus (ACLSV) genomic RNA has been determined from cDNA clones.
This sequence contains three putative open reading frames (ORFs) capable of encoding proteins of 216.5, 50, and 28 kDa.
The 216.5 kDa ORF encodes a protein which contains the conserved "signature" sequences and has significant homology with the proteins suspected to be involved in viral replication of the members of the "Alpha-like" supergroup of viruses.
The 50 kDa ORF is suspected to encode a protein responsible for virus cell-to-cell spread.
The 28 kDa ORF contains, in frame, a smaller 21.5 kDa ORF encoding the coat protein of ACLSV.

Double stranded RNAs (dsRNAs) were isolated from plants infected with five different strains or isolates of ACLSV, differing by their original host, geographical origin or symptomatology.
Analysis by polyacrylamide gel electrophoresis showed that 3 major species of viral dsRNAs can be detected in all the infected plants, irrespective of the ACLSV strain used.
Furthermore, preliminary northern blot hybridization experiments showed that in addition to these three dsRNAs, two smaller dsRNA species can also be detected, which could correspond to the double stranded forms of two subgenomic messengers RNAs for the 50 kDa protein and the coat protein.

XV International Symposium on Fruit Tree Diseases

S. German, T. Candresse, M. Lanneau, J. Dunez

https://doi.org/10.17660/ActaHortic.1992.309.2