Home > XV International Symposium on Fruit Tree Diseases > ENZYMATIC CDNA AMPLIFICATION OF HOP STUNT VIROID VARIANTS FROM NATURALLY INFECTED FRUIT CROPS

Articles

ENZYMATIC CDNA AMPLIFICATION OF HOP STUNT VIROID VARIANTS FROM NATURALLY INFECTED FRUIT CROPS

Article number

309_49

Pages

339 – 344

Language

Abstract

Hop stunt viroid (HSV) cDNA reverse transcribed in vitro from total nucleic acid extracts of infected tissue was amplified using Taq I DNA polymerase and HSV group-specific DNA primers in a reverse transcription-polymerase chain reaction (RT-PCR). The RT-PCR assay is a rapid and specific method to determine HSV infection in fruit crops without viroid purification or the need for large amounts of infected tissue.

Publication

XV International Symposium on Fruit Tree Diseases

Authors

A. Hadidi, Y. Terai, C.A. Powell, S.W. Scott, J.C. Desvignes, L.M. Ibrahim, L. Levy

Keywords

Detection, Polymerase chain reaction (PCR), Prunus, Viroids

Full text

https://doi.org/10.17660/ActaHortic.1992.309.49

Online Articles (60)

2. TREE FRUIT VIRUS DISEASES – WHERE ARE WE HEADING?

A.J. HANSEN

3. GENOMIC ORGANIZATION OF APPLE CHLOROTIC LEAF SPOT CLOSTEROVIRUS (ACLSV)

S. German | T. Candresse | M. Lanneau | J. Dunez

4. PARTIAL CHARACTERIZATION OF A CLOSTEROVIRUS-LIKE PARTICLE TRANSMITTED FROM CHERRY (PRUNUS AVIUM) INFECTED WITH SEVERAL VIRAL DISEASES

D. JAMES

5. PROPERTIES AND TRANSLATION STUDIES OF CHERRY LEAF ROLL VIRUS (CLRV)

V. Pallas | A. Rowhani | S. Daubert | G. Bruening

6. CDNA CLONING OF DSRNA ASSOCIATED WITH APPLE STEM PITTING DISEASE AND EVIDENCE FOR THE RELATIONSHIP OF THE VIRUS-LIKE AGENTS ASSOCIATED WITH APPLE STEM PITTING AND PEAR VEIN YELLOWS

W. Jelkmann | L. Kunze | H.-J. Vetten | D.-E. Lesemann

7. STUDY ON PRUNUS NECROTIC RING SPOT VIRUS IN GREECE

C. Varveri

8. EVIDENCE FOR THE SPREAD OF PRUNUS NECROTIC RINGSPOT VIRUS AND THE PRESENCE OF PRUNE DWARF VIRUS IN SELECTED PEACH ORCHARDS IN SOUTH CAROLINA

S.W. Scott | R. Walker Miller | E. J. Bachman

9. THE USE OF NUCLEIC ACID PROBES FOR THE DETECTION OF PRUNUS NECROTIC RINGSPOT VIRUS AND PRUNE DWARF VIRUS

S.W. Scott | V. Bowman-Vance | E. J. Bachman

10. COMPARATIVE BIOLOGICAL, SEROLOGICAL AND ELECTROPHORETIC BEHAVIOUR OF SOME ISOLATES OF PRUNUS NECROTIC RINGSPOT ILARVIRUS

J. Tuzovic | M. Digiaro

11. ILAVIRUSES IN APRICOT AND PLUM POLLEN

M. Digiaro | V. Savino | B. Di Terlizzi

12. DETECTION OF PRUNUS NECROTIC RINGSPOT VIRUS BY BIOASSAY AND IEM TECHNIQUE DURING THE VEGETATION PERIOD OF SOUR CHERRY TREES

S. Kryczynski | M.S. Szyndel | A. Stawiszynska | A. Andersz-Wroblewska

13. THE RATE AND WAY OF PRUNUS NECROTIC RINGSPOT VIRUS SPREAD IN SOUR CHERRY ORCHARD AND IN THE ROOTSTOCK PRODUCTION

S. Kryczynski | M.S. Szyndel | A. Stawiszynska | W. Piskorek

14. EFFECTS OF TOMATO RINGSPOT VIRUS AND PRUNUS NECROTIC RINGSPOT VIRUS ALONE AND IN COMBINATION ON THE GROWTH AND YIELD OF ‘MONTMORENCY’ SOUR CHERRY

D.C. Ramsdell | G.W. Bird | V.A. Adler | J.M. Gillett

15. CHERRY RASP LEAF ETIOLOGY IN CAMPANIA

A. Ragozzino | D. Alioto

16. OCCURRANCE OF PLUM POX (SHARKA) VIRUS ON STONE FRUIT TREES IN EGYPT

H.M. Mazyad | M.K. Nakhla | A. Abo-Elela | M.H. El-Hammady

17. PLUM POX VIRUS OUTBREAKS IN APULIA AND BASILICATA (SOUTHERN ITALY)

V. Savino | M. Digiaro | G.P. Martelli | B. Di Terlizzi

18. EPIDEMIOLOGY OF PLUM POX (SHARKA) VIRUS IN VALENCIA (SPAIN)

G. Llácer | L. Avinent | A. Hermoso de Mendoza

19. INVESTIGATIONS ON THE SPREAD OF SHARKA IN VIEW OF A SYSTEMATIC VIRUS CONTROL

M. Keck | A. Stöger | K. Russ

20. THE PRODUCTION OF SHARKA FREE PROPAGATING MATERIAL OF APRICOTS AND PEACHES IN CZECH REPUBLIC

J. Polák | I. Oukropec | J. Chod

21. STUDY OF THE ECOLOGICAL FACTORS INFLUENCING ATTACKS BY PLUM POX VIRUS

G. Vanek | J. Kotuc | M. Kölber | M. Németh | L. Szõke

22. EVALUATION OF PRUNUS SPINOSA L. AS HOST OF SHARKA AND OTHER VIRUSES

M. Rankovic | I. Dulic-Markovic

23. THE SUITABILITY OF MODIFIED ELISA FOR DETECTION OF PLUM POX VIRUS.

T. Malinowski | B. Zawadzka

24. DIAGNOSIS OF PLUM POX VIRUS BY ELISA

I. Tóbiás | K. Gyozo | M. Kölber

25. MONOCLONAL ANTIBODIES AGAINST PLUM POX VIRUS

M. Navrátil | D. Cikánek | I. Hilgert | H. Krištofová | R. Karešová

26. MOLECULAR TECHNIQUES FOR PLUM POX POTYVIRUS (PPV) DETECTION AND CLONING OF THE GENOMIC RNA FROM AN ATYPICAL STRAIN: PPV EL AMAR

27. TRANSFORMATION AND REGENERATION OF PLANTS OF PRUNUS ARMENIACA WITH THE COAT PROTEIN GENE OF PLUM POX VIRUS

28. TRANSGENIC TOBACCO PLANTS THAT CONTAIN THE PLUM POX VIRUS (PPV) COAT PROTEIN GENE.

M. Ravelonandro | M. Monsion | P.-Y. Teycheney | R.P. Delbos | J. Dunez

29. IN VITRO AND IN VIVO EXPRESSION OF PPV HELPER-COMPONENT GENE

M. Ravelonandro | G. Tavert-Roudet | L. Garrigue | O. Peyruchaud | J. Dunez

30. COAT PROTEIN-MEDIATED PROTECTION AGAINST PLUM POX VIRUS

31. EVALUATION OF RESISTANCE TO PLUM POX VIRUS IN APRICOT TREES

32. EVALUATION OF PEACH AND NECTARINE VARIETIES ACCORDING TO RESISTANCE TO SHARKA (PLUM POX) VIRUS

A.Ch. Mainou | G.D. Syrgianidis

33. THE RESISTANCE OF THE PLUM HYBRID K4 AND ITS PROGENIES TO PLUM POX VIRUS

H. Kegler | M. Grüntzig

34. APPLE DECLINE

C.L. PARISH | Dr. Eric Seemuller | B.C. Kirkpatrick

35. APPLE DECLINE IN BRAZIL

C.H. de Souza | C.L. Parish

36. RESISTANCE IN ESTABLISHED AND EXPERIMENTAL APPLE ROOTSTOCKS TO APPLE PROLIFERATION DISEASE

E. Seemüller | S. Kartte | L. Kunze

37. DISTRIBUTION OF APPLE PROLIFERATION DISEASE IN ORCHARDS WITH INTEGRATED AND CONVENTIONAL PESTICIDE PROGRAMS

G. Krczal | K. Bliefernicht

38. ABSENCE OF DECLINE-LIKE SYMPTOMS IN ENGLISH PEAR TREES PROTECTED FROM EARLY SEASON POPULATIONS OF CACOPSYLLA PYRICOLA

C.M. Guise | D.L. Davies

39. INVESTIGATIONS ON PEAR FRUIT ATROPHY

N. Minoiu | M. Ionica

40. THE ELIMINATION OF MYCOPLASMA-LIKE ORGANISMS FROM PEAR BUDWOOD BY HOT WATER TREATMENT

A.N. ADAMS | D.L. DAVIES

41. PRODUCTION AND CHARACTERIZATION OF POLYCLONAL AND MONOCLONAL ANTIBODIES AGAINST PEACH YELLOW LEAFROLL MLO-ASSOCIATED ANTIGENS

D.L. Davies | M.F. Clark

42. NATURAL DIFFUSION AND EXPERIMENTAL TRANSMISSION OF PLUM “LEPTONECROSIS”(*)

L. Carraro | R. Osler | E. Refatti | M.A. Favali

43. RAPID BIOLOGICAL DETECTION OF APPLE SCAR SKIN VIROID

W.E. Howell | G.I. Mink

44. APPLE SCAR SKIN AND DAPPLE APPLE VIROIDS ARE SEED BORNE

A. Hadidi | A.J. Hansen | C.L. Parish

45. NUCLEOTIDE SEQUENCE OF APPLE SCAR SKIN VIROID REVERSE TRANSCRIBED IN HOST EXTRACTS AND AMPLIFIED BY THE POLYMERASE CHAIN REACTION

X. Yang | A. Hadidi | R.W. Hammond

46. EARLY DETECTION OF APPLE SCAR SKIN GROUP VIROIDS FROM IMPORTED PEAR GERMPLASM

S.S. Hurtt | E.V. Podleckis | A. Hadidi | L.M. Ibrahim

47. EVIDENCES SUPPORTING A VIROID ETIOLOGY FOR PEAR BLISTER CANKER DISEASE

C. Hernández | R. Flores | G. Llácer | J.C. Desvignes

48. STUDIES ON THE DETECTION, TRANSMISSION AND DISTRIBUTION OF PEACH LATENT MOSAIC VIROID IN PEACH TREES

49. PEACH LATENT MOSAIC VIROID IN ITALY

G. Albanese | L. Giunchedi | R. La Rosa | C. Poggi Pollini

50. ENZYMATIC CDNA AMPLIFICATION OF HOP STUNT VIROID VARIANTS FROM NATURALLY INFECTED FRUIT CROPS

51. OCCURRENCE OF PETUNIA ASTEROID MOSAIC (PAMV) AND CARNATION ITALIAN RINGSPOT (CIRV) VIRUSES IN CHERRY ORCHARDS IN NORTHERN BAVARIA

E. Pfeilstetter | V. Zinkernagel | L. Kunze

52. CHARACTERIZATION OF THE CHESTNUT MOSAIC

J.C. Desvignes

53. APRICOT RING-POX-LIKE DISEASE AND RELATED DISORDERS OF APRICOT FRUITS IN CAMPANIA

A. Ragozzino | D. Alioto | A. Zoina

54. UNUSUAL FRUIT DISORDERS IN TIRYNTHOS APRICOT

B. Di Terlizzi | V. Savino | M. Digiaro

55. VIRUSES OF PEACH, PLUM AND APRICOT IN APULIA

B. Di Terlizzi | O. Murolo | V. Savino | M. Digiaro

56. CITRUS RING PATTERN IN THE CASPIAN SEA AREA OF IRAN

M. HABASHI

57. QUICK DETECTION OF THE PRINCIPAL APPLE AND PEAR VIRUS DISEASES

J.C. Desvignes | R. Boyé | D. Cornaggia | N. Grasseau

58. COMPARISON OF DIFFERENT METHODS TO PRODUCE VIRUS FREE STONE FRUITS

M. Barba | L. Martino | F. Lauretti

59. RECOVERY OF VIRUS-FREE ALMOND PLANTS BY SHOOT-TIP GRAFTING IN VITRO

60. THERMOTHERAPY AND INDEXING : A SYNTHESIS OF SOME RESULTS OBTAINED AT THE RESEARCH STATION OF GORSEM

P.J. Simon | G.A. Peusens | G.L. Gilles | H. Bormans